Tilapia Lake Virus (TiLV): a Globally Emerging Threat to Tilapia Aquaculture
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Tilapia Lake Virus TiLV

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Al-Hussinee, Lowia, Kuttichantran Subramaniam, Win Surachetpong, Vsevolod Popov, Kathleen Hartman, Katharine Starzel, Roy Yanong, Craig Watson, Hugh Ferguson, Salvatore Frasca Jr, and Thomas Waltzek. 2019. “Tilapia Lake Virus (TiLV): A Globally Emerging Threat to Tilapia Aquaculture: FA213, 4/2019”. EDIS 2019 (2). Gainesville, FL. https://doi.org/10.32473/edis-fa213-2019.


Tilapia lake virus is a globally emerging virus responsible for episodes of mass mortality in cultured and/or feral tilapia (Oreochromis spp. and hybrids) in Asia, Africa, Central America, and South America. Since 2014, there have been global reports of TiLV disease resulting in 10% to 90% mortality in tilapia fry, juveniles, and adults causing significant economic losses. Currently, the disease has been confirmed in Colombia, Ecuador, Egypt, India, Indonesia, Israel, Malaysia, Mexico, Philippines, Peru, Tanzania, and Thailand. TiLV has not yet been found in the USA or Canada, but it has most recently been reported in 20 aquaculture production facilities across six Mexican states (Chiapas, Jalisco, Michoacán, Sinaloa, Tabasco and Veracruz). This 7-page fact sheet written by Lowia Al-Hussinee, Kuttichantran Subramaniam, Win Surachetpong, Vsevolod Popov, Kathleen Hartman, Katharine Starzel, Roy Yanong, Craig Watson, Hugh Ferguson, Salvatore Frasca Jr., and Thomas Waltzek and published by the UF/IFAS School of Forest Resources and Conservation, Program in Fisheries and Aquatic Sciences describes this important emerging disease and explains how to prevent outbreaks and what to do if you suspect TiLV in an aquaculture facility or in the wild. 

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Adams, M J., E. L. Lefkowitz, A. M. Q. King, B. Harrach, R. L. Harrison, N J., Kropinski, M. Krupovic, J. H. Kuhn, A. R. Mushegian, M. Nibert, S. Sabanadzovic, H. Sanfacon, S. G. Siddell, P. Simmonds, A. Varsani, F. M. Zerbini, A. E. Gorbalenya, A. J. Davison. 2017. Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses. Arch Virology 162: 2505-2538. https://doi.org/10.1007/s00705-017-3358-5

Al-Hussinee, L., K. Subramaniam, M. S. Ahasan, B. Keleher, and T. Waltzek. 2018. "Complete Genome Sequence of Tilapia Lake Virus Isolated from Nile Tilapia (Oreochromis niloticus)." Genome Announcements 6:e00580-18. https://doi.org/10.1128/genomeA.00580-18

Bacharach, E., N. Mishra, T. Briese, A. Eldar, WI. Lipkin, JH. Kuhn. 2016. "ICTV taxonomic proposal 2016.016a-dM.A.v2.Tilapinevirus. Create the unassigned genus Tilapinevirus. Accessed April 4 2017. Available online at: https://talk.ictvonline.org/ICTV/proposals/2016.016a-dM.A.v2.Tilapinevirus.pdf

Bacharach, E., N. Mishra, T. Briese, M. C. Zody, J. E. K. Tsofack, R. Zamostiano, A. Berkowitz, J. Ng, A. Nitido, and A. Corvelo. 2016. "Characterization of a novel orthomyxo-like virus causing mass die-offs of tilapia." mBio 7:e00431-16. https://doi.org/10.1128/mBio.00431-16

Behera, B. K., P. K. Pradhan, T. T. Swaminathan, N. Sood, P. Paria, A. Das, D. K. Verma, R. Kumar, M. K. Yadav, A. K. Dev, P. K. Parida, B. K. Das, K. K. Lal, and J. K. Jena. 2018. "Emergence of Tilapia Lake Virus associated with mortalities of framed Nile Tilapia Oreochromis niloticus (Linneaus 1758) In India." Aquaculture 484:168-174. https://doi.org/10.1016/j.aquaculture.2017.11.025

Del-Pozo J., N. Mishra, R. Kabuusu, S. Cheetham, A. Eldar, E. Bacharach, W. I. Lipkin, and H. W. Ferguson. 2017. "Syncytial hepatitis of tilapia (Oreochromis niloticus L.) is associated with orthomyxovirus-like virions in hepatocytes." Veterinary Pathology 54:164-170. https://doi.org/10.1177/0300985816658100

Dong, H., T. Rattanarojpong, and S. Senapin. 2017. "Urgent Update on Possible Worldwide Spread of Tilapia Lake Virus (TiLV)." Network of Aquaculture Centres In Asia. Accessed January 10, 2018. Available online at: https://enaca.org/?id=870&title=urgent-update-on-possible-worldwide-spread-of-tilapia-lake-virus-tilv

El-Sayed, A. F. M. 2006. Tilapia Culture. CABI Publishing, Cambridge, Massachusetts. https://doi.org/10.1079/9780851990149.0000

Eyngor, M., R. Zamostiano, J. E. K. Tsofack, A. Berkowitz, H. Bercovier, S. Tinman, M. Lev, A. Hurvitz, M. Galeotti, and E. Bacharach. 2014. "Identification of a novel RNA virus lethal to tilapia." Journal of Clinical Microbiology 52:4137-4146. https://doi.org/10.1128/JCM.00827-14

FAO. 2017. "Global Aquaculture Production." Food and Agriculture Organization of the United Nations, Rome. Accessed December 20, 2017. Available online at: http://www.fao.org/fishery/statistics/global-production/en

Fathi, M., C. Dickson, M. Dickson, W. Leschen, J. Baily, F. Muir, K. Ulrich, and M. Weidmann. 2017. "Identification of Tilapia Lake Virus in Egypt in Nile tilapia affected by 'summer mortality' syndrome." Aquaculture 473:430-432. https://doi.org/10.1016/j.aquaculture.2017.03.014

Ferguson, H., R. Kabuusu, S. Beltran, E. Reyes, J. Lince, and J. Del Pozo. 2014. "Syncytial hepatitis of farmed tilapia, Oreochromis niloticus (L.): a case report." Journal of Fish Diseases 37:583-589. https://doi.org/10.1111/jfd.12142

Francis-Floyd, R. 1990. Stress: Its Role in Fish Disease. Gainesville: University of Florida Institute of Food and Agricultural Sciences. Accessed January 06, 2018. Available online at: https://edis.ifas.ufl.edu/fa005

Jaemwimol, P., P. Rawiwan, P. Tattiyapong, P. Saengnual, A. Kamlangdee, and W. Surachetpong. 2018. "Susceptibility of important warm water fish species to tilapia lake virus (TiLV) infection." Aquaculture 497:462-468. https://doi.org/10.1016/j.aquaculture.2018.08.028

Jansen, M. D., H. T. Dong, and C. V. Mohan. 2018. "Tilapia lake virus: a threat to the global tilapia industry?" Reviews in Aquaculture In Press. doi: 10.1111/raq.12254. https://doi.org/10.1111/raq.12254

Kabuusu, R. M., A. T. Aire, D. F. Stroup, C. N. L. Macpherson, and H. W. Ferguson. 2018. "Production-level risk factors for syncytial hepatitis in farmed tilapia (Oreochromis niloticus L)." Journal of Fish Diseases 41:61-66. https://doi.org/10.1111/jfd.12672

Kembou Tsofack, J. E. K., R. Zamostiano, S. Watted, A. Berkowitz, E. Rosenbluth, N. Mishra, T. Briese, W. I. Lipkin, R. M. Kabuusu, and H. W. Ferguson. 2017. "Detection of Tilapia lake virus in clinical samples by culturing and nested reverse transcription-PCR." Journal of Clinical Microbiology 55:759-767. https://doi.org/10.1128/JCM.01808-16

Koesharyani, I., L. Gardenia, Z. Widowati, K. Khumaira, and D. Rustianti. 2018. "Studi kasus infeksi Tilapia lake virus (TiLV) pada ikan nila (Oreochromis niloticus)." Jurnal Riset Akuakultur 13:85-92. https://doi.org/10.15578/jra.13.1.2018.85-92

Liamnimitr, P., W. Thammatorn, S. U-Thoomporn, P. Tattiyapong, and W. Surachetpong. 2018. "Non-lethal sampling for Tilapia Lake Virus detection by RT-qPCR and cell culture." Aquaculture 486:75-80. https://doi.org/10.1016/j.aquaculture.2017.12.015

Mugimba, K., A. Chengula, S. Wamala, E. Mwega, C. Kasanga, D. Byarugaba, R. Mdegela, S. Tal, B. Bornstein, and A. Dishon. 2018. "Detection of tilapia lake virus (Ti LV) infection by PCR in farmed and wild Nile tilapia (Oreochromis niloticus) from Lake Victoria." Journal of Fish Diseases 41:1181-1189. https://doi.org/10.1111/jfd.12790

Nicholson, P., M. Fathi, A. Fischer, C. Mohan, E. Schieck, N. Mishra, A. Heinimann, J. Frey, B. Wieland, and J. Jores. 2017. "Detection of Tilapia Lake Virus in Egyptian fish farms experiencing high mortalities in 2015." Journal of Fish Diseases 40:1925-1928. https://doi.org/10.1111/jfd.12650

OIE. 2017a. "Tilapia Lake Virus Disease, Malaysia." World Organisation for Animal Health (OIE). Immediate Notification. Accessed January 10, 2018. Available online at: https://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=24809

OIE. 2017b. "Tilapia Lake Virus (TiLV), Philippines." World Organisation for Animal Health (OIE). Immediate Notification. Accessed January 10, 2018. Available online at: http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=25278

OIE. 2018a. "Tilapia Lake Virus (TiLV), Mexico." World Organisation for Animal Health (OIE). Immediate Notification. Accessed January 10, 2018. Available online at: https://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=27650

OIE. 2018b. "Tilapia Lake Virus (TiLV), Peru." World Organisation for Animal Health (OIE). Immediate Notification. Accessed January 10, 2018. Available online at: http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=26027

Plumb, J. A., and L. A. Hanson. 2011. "Health maintenance and principal microbial diseases of cultured fishes." 3rd ed. Wiley-Blackwell, Ames, Iowa.

Shlapobersky, M., M. S. Sinyakov, M. Katzenellenbogen, R. Sarid, J. Don, and R. R. Avtalion. 2010. "Viral encephalitis of tilapia larvae: Primary characterization of a novel herpes-like virus." Virology 399:239-247. https://doi.org/10.1016/j.virol.2010.01.001

Subramaniam K., H.W. Ferguson, and T.B. Waltzek. 2019. "Genome Sequence of Tilapia Lake Virus Associated With Syncytial Hepatitis of Tilapia in an Ecuador Aquaculture Facility." Microbiology Resource Announcement In press. https://doi.org/10.1128/MRA.00084-19

Subramaniam, K., M. Gotesman, C. E. Smith, N. K. Steckler, K. L. Kelley, J. M. Groff, and T. B. Waltzek. 2016. Megalocytivirus infection in cultured Nile tilapia Oreochromis niloticus. Diseases of Aquatic Organisms 119:253-258. https://doi.org/10.3354/dao02985

Surachetpong, W., T. Janetanakit, N. Nonthabenjawan, P. Tattiyapong, K. Sirikanchana, and A. Amonsin. 2017. "Outbreaks of tilapia lake virus infection, Thailand, 2015-2016." Emerging Infectious Diseases 23:1031-1033. https://doi.org/10.3201/eid2306.161278

Tattiyapong, P., W. Dachavichitlead, and W. Surachetpong. 2017. "Experimental infection of Tilapia Lake Virus (TiLV) in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.)." Veterinary Microbiology 207:170-177. https://doi.org/10.1016/j.vetmic.2017.06.014

Tattiyapong, P., K. Sirikanchana, and W. Surachetpong. 2018. "Development and validation of a reverse transcription quantitative polymerase chain reaction for Tilapia Lake Virus detection in clinical samples and experimentally challenged fish." Journal of Fish Diseases 41:255-261. https://doi.org/10.1111/jfd.12708

Thangaraj, R. S., C. Ravi, R. Kumar, A. Dharmaratnam, B. V. Saidmuhammed, P. K. Pradhan, and N. Sood. 2018. "Derivation of two tilapia (Oreochromis niloticus) cell lines for efficient propagation of Tilapia Lake Virus (TiLV)." Aquaculture 492:206-214. https://doi.org/10.1016/j.aquaculture.2018.04.012

Watanabe, W.O., T. M. Losordo, K. Fitzsimmons, and F. Hanley. 2002. "Tilapia production systems in the Americas: Technological advances, trends, and challenges." Reviews in fisheries science 10:465-498. https://doi.org/10.1080/20026491051758

Yanong, R.P.E. 2013. "Biosecurity in Aquaculture, Part 3: Ponds." USDA-Southern Regional Aquaculture Center. SRAC publication No. 4712. Accessed January 6, 2018. Available online at: https://agrilifecdn.tamu.edu/fisheries/files/2013/09/SRAC-Publication-No.-4712-Biosecurity-in-Aquaulture-Part-3-Ponds.pdf

Yanong, R.P.E., and C. Erlacher-Reid. 2012. "Biosecurity in Aquaculture, Part 1: An Overview." USDA-Southern Regional Aquaculture Center. SRAC publication No. 4707. Accessed January 6, 2018. Available online at: http://fisheries.tamu.edu/files/2013/09/SRAC-Publication-No.-4707-Biosecurity-in-Aquaculture-Part-1-An-Overview.pdf

Yanong, R.P.E. 2012. "Biosecurity in Aquaculture, Part 2: Recirculating Aquaculture Systems." USDA-Southern Regional Aquaculture Center. SRAC Publication No. 4708. Accessed January 6, 2018. Available online at: https://agrilifecdn.tamu.edu/fisheries/files/2013/09/SRAC-Publication-No.-4708-Biosecurity-in-Aquaculture-Part-2-Recirculating-Aquaculture-Systems.pdf

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