A Semen Extender for the Short-Term Storage of Fish Sperm
Male (left) and female (right) Gulf killifish, Fundulus grandis.
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Keywords

Fish Reproduction
FA193

Categories

How to Cite

Chapman, Frank A. 2016. “A Semen Extender for the Short-Term Storage of Fish Sperm: FA193/FA193, 5/2016”. EDIS 2016 (5). Gainesville, FL:3. https://doi.org/10.32473/edis-fa193-2016.

Abstract

Aquaculturists worldwide use artificial or induced spawning of fish to maximize egg and larval production from fish that cannot normally be bred in captivity. Despite the wide global use of this technique, and much literature published, the success rates of induced spawning are consistently variable. One often overlooked reason for the variable success rates is that successful rates of fertilization, hatching, and larval survival are most dependent on high-quality sperm and the surrounding fluid that supports sperm function. It is difficult to obtain consistent, good-quality spermiations (releases of spermatozoa); to keep sperm alive after collection and during storage and transport; and to freeze large volumes of semen at one time. Therefore, a successful fish breeding program requiring sperm begins with a source of high-quality semen, and its proper collection, handling, and storage. This 3-page article describes how to make and use a semen extender that will maximize the volume and preserve the viability of obtained semen. Written by Frank A. Chapman and published by the Program in Fisheries and Aquatic Sciences in the School of Forest Resources and Conservation, May 2016.

FA193/FA193: A Semen Extender for the Short-Term Storage of Fish Sperm (ufl.edu)

https://doi.org/10.32473/edis-fa193-2016
view on EDIS
PDF-2016

References

Ciereszko, A., and K. Dabrowski. 1994. "Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage." Fish Physiology and Biochemistry 12(5): 357-367. https://doi.org/10.1007/BF00004300

Lahnsteiner, F., T. Weismann, and R. A. Patzner. 1997. "Aging processes of rainbow trout semen during storage." The Progressive Fish-Culturist 59(4): 272-279. https://doi.org/10.1577/1548-8640(1997)059<0272:APORTS>2.3.CO;2

Li, J., Q. Liu, and S. Zhang. 2006. "Evaluation of the damage in fish spermatozoa cryopreservation." Chinese Journal of Oceanology and Limnology 24(4): 370-377. https://doi.org/10.1007/BF02842852

Park, C., and F. A. Chapman. 2005. "An extender solution for the short-term storage of sturgeon semen." North American Journal of Aquaculture 67: 52-57. https://doi.org/10.1577/FA03-068.1

Stoss, J. 1983. "Fish gamete preservation and spermatozoa physiology." Pp. 305-350. In: Hoar WS, Randall DJ, Donaldson EM, eds. Fish Physiology, Volume 9B. Academic Press. https://doi.org/10.1016/S1546-5098(08)60306-4

Tiersch, T. R., and P. M. Mazik. 2000. Cryopreservation in aquatic species. Advances in World Aquaculture, Volume 7. The World Aquaculture Society.
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