Abstract
The dairy cow is unique because virtually all cows are infected with bacteria right after calving. A recent study that used metagenomic analysis to characterize the uterine flora in healthy and metritic cows observed that most clone sequences from the metritic cows were from the phylum Fusobacteria. This 4-page fact sheet summarizes what is known about the pathogenic bacteria associated with metritis and endometritis. Written by Klibs N. Galvão and published by the UF Department of Veterinary Medicine-Large Animal Clinical Sciences, January 2012.
VM182/VM182: Uterine Microbiology of Healthy Cows and Cows That Develop Uterine Disease (ufl.edu)
References
Bicalho, R.C., V.S. Machado, M.L. Bicalho, R.O. Gilbert, A.G. Teixeira, L.S. Caixeta, and R.V. Pereira. 2010. "Molecular and Epidemiological Characterization of Bovine Intrauterine Escherichia Coli." J. Dairy Sci. 93: 5818-30. https://doi.org/10.3168/jds.2010-3550
Bondurant, R.H. 1999. "Inflammation in the Bovine Female Reproductive Tract." J Anim Sci. 77 (Suppl 2): 101-10. https://doi.org/10.2527/1999.77suppl_2101x
Bonnett, B.N., S.W. Martin, V.P. Gannon, R.B. Miller, and W.G. Etherington. 1991. "Endometrial Biopsy in Holstein-Friesian Dairy Cows III. Bacterial Analysis and Correlations with Histological Findings." Can. J. Vet. Res. 55: 168-73.
Dohmen, M. J., K. Joop, A. Sturk, P. E. Bols, and A. Lohuis. 2000. "Relationship between Intra-uterine Bacterial Contamination, Endotoxin Levels and the Development of Endometritis in Postpartum Cows with Dystocia or Retained Placenta." Theriogenology 54: 1019-32. https://doi.org/10.1016/S0093-691X(00)00410-6
Donofrio, G., S. Herath, C. Sartori, S. Cavirani, C.F. Flammini, and I.M. Sheldon. 2007. "Bovine Herpesvirus 4 Is Tropic for Bovine Endometrial Cells and Modulates Endocrine Function." Reproduction 134: 183-97. https://doi.org/10.1530/REP-07-0065
Donofrio, G., L. Ravanetti, S. Cavirani, S. Herath, A. Capocefalo, and I.M. Sheldon. 2008. "Bacterial Infection of Endometrial Stromal Cells Influences Bovine Herpesvirus 4 Immediate Early Gene Activation: A New Insight into Bacterial and Viral Interaction for Uterine Disease." Reproduction 136: 361-6. https://doi.org/10.1530/REP-08-0171
Donofrio, G., V. Franceschi, A. Capocefalo, S. Cavirani, and I.M. Sheldon. 2009. "Isolation and Characterization of Bovine Herpesvirus 4 (BoHV-4) from a Cow Affected by Post-partum Metritis and Cloning of the Genome as a Bacterial Artificial Chromosome." Reprod. Biol. Endocrinol.19: 83. https://doi.org/10.1186/1477-7827-7-83
Donofrio, G., A. Capocefalo, V. Franceschi, S. Price, S. Cavirani, and I.M. Sheldon. 2010. "The Chemokine IL8 Is Up-regulated in Bovine Endometrial Stromal Cells by the BoHV-4 IE2 Gene Product, ORF50/Rta: A Step Ahead toward a Mechanism for BoHV-4 Induced Endometritis." Biol. Reprod. 83: 919-28. https://doi.org/10.1095/biolreprod.110.086074
Elliot, L., K.J. McMahon, H.T. Gier, and G.B. Marion. 1968. "Uterus of the Cow after Parturition: Bacterial Content." Am. J. Vet. Res. 29: 77-81.
Farin, P.W., L. Ball, J.D. Olson, R.G. Mortimer, R.L. Jones, W.S. Adney, and A.E. McChesney. 1989. "Effect of Actinomyces Pyogenes and Gram-negative Anaerobic Bacteria on the Development of Bovine Pyometra." Theriogenology 31: 979-89. https://doi.org/10.1016/0093-691X(89)90481-0
Földi, J., M. Kulcsár, A. Pécsi, B. Huyghe, C. de Sa, J.A. Lohuis, P. Cox, and G. Huszenicza. 2006. "Bacterial Complications of Postpartum Uterine Involution in Cattle." Anim. Reprod. Sci. 96: 265-81. https://doi.org/10.1016/j.anireprosci.2006.08.006
Frazier, K., M. Pence, M.J. Mauel, A. Liggett, M.E. Hines II, L. Sangster, H.D. Lehmkuhl, D. Miller, E. Styer, J. West, and C.A. Baldwin. 2001. "Endometritis in Postparturient Cattle Associated with Bovine Herpesvirus-4 Infection: 15 Cases." J. Vet. Diagn. Invest. 13: 502-8. https://doi.org/10.1177/104063870101300608
Galvão, K.N., L.F. Greco, J.M. Vilela, M.F. Sá Filho, and J.E. Santos. 2009. "Effect of Intrauterine Infusion of Ceftiofur on Uterine Health and Fertility in Dairy Cows." J. Dairy Sci. 92: 1532-42. https://doi.org/10.3168/jds.2008-1615
Gilbert, R.O., N.R. Santos, K.N. Galvão, S.B. Brittin, and H.B. Roman. 2007. "The Relationship between Postpartum Uterine Bacterial Infection (BI) and Subclinical Endometritis (SE)." J. Dairy Sci. 90(Suppl. 1): 469 (Abstr).
Griffin, J.F.T., P.J. Hartigan, and W.R. Nunn. 1974. "Non-specific Uterine Infection and Bovine Fertility I. Infection Patterns and Endometritis during the First Seven Weeks Post-partum." Theriogenology 1: 91-106. https://doi.org/10.1016/0093-691X(74)90052-1
Huszenicza, G., M. Fodor, M. Gacs, M. Kulscar, M.J. Dohmen, M. Vamos, L. Portokolab, T. Kegl, J. Bartyik, J.C. Janosi, and G. Szita. 1999. "Uterine Bacteriology, Resumption of Ovarian Activity and Fertility in Postpartum Cows Kept in Large-scale Dairy Herds." Reprod. Domest. Anim. 34: 237-45. https://doi.org/10.1111/j.1439-0531.1999.tb01246.x
Mateus, L., L.L. da Costa, F. Bernardo, and J.R. Silva. 2002. "Influence of Puerperal Uterine Infection on Uterine Involution and Postpartum Ovarian Activity in Dairy Cows." Reprod. Domest. Anim. 37: 31-5. https://doi.org/10.1046/j.1439-0531.2002.00317.x
Miller, A.N., E.J. Williams, K. Sibley, S. Herath, E.A. Lane, J. Fishwick, D.M. Nash, A.N. Rycroft, H. Dobson, C.E. Bryant, and I.M. Sheldon. 2007. "The Effects of Arcanobacterium pyogenes on Endometrial Function in Vitro, and on Uterine and Ovarian Function in Vivo." Theriogenology 68: 972-80. https://doi.org/10.1016/j.theriogenology.2007.07.013
Monge, A., L. Elvira, J.V. Gonzalez, S. Astiz, and G.J. Wellenberg. 2006. "Bovine Herpesvirus 4-associated Postpartum Metritis in a Spanish Dairy Herd." Res. Vet. Sci. 80:120-5. https://doi.org/10.1016/j.rvsc.2005.04.001
Olson, J.D., L. Ball, R.G. Mortimer, P.W. Farin, W.S. Adney, and E.M. Huffman. 1984. "Aspects of Bacteriology and Endocrinology of Cows with Pyometra and Retained Fetal Membranes." Am. J. Vet. Res. 45: 2251-5.
Ruder, C.A., R.G. Sasser, R.J. Williams, J.K. Ely, R.C. Bull, and J.E. Butler. 1981. "Uterine Infections in the Postpartum Cow. II. Possible Synergistic Effect of Fusobacterium necrophorum and Corynebacterium pyogenes." Theriogenology 15: 573-580. https://doi.org/10.1016/0093-691X(81)90060-1
Santos, T.M., L.S. Caixeta, V.S. Machado, A.K. Rauf, R.O. Gilbert, and R.C. Bicalho. 2010. "Antimicrobial Resistance and Presence of Virulence Factor Genes in Arcanobacterium pyogenes Isolated from the Uterus of Postpartum Dairy Cows." Vet. Microbiol. 145: 84-9. https://doi.org/10.1016/j.vetmic.2010.03.001
Santos, T.M., R.O. Gilbert, and R.C. Bicalho. 2011. "Metagenomic Analysis of the Uterine Bacterial Microbiota in Healthy and Metritic Postpartum Dairy Cows." J. Dairy Sci. 94: 291-302. https://doi.org/10.3168/jds.2010-3668
Sheldon, I.M., D.E. Noakes, A.N. Rycroft, D.U. Pfeiffer, and H. Dobson. 2002. "Influence of Uterine Bacterial Contamination after Parturition on Ovarian Dominant Follicle Selection and Follicle Growth and Function in Cattle." Reproduction. 123: 837-45. https://doi.org/10.1530/rep.0.1230837
Sheldon, I.M., and H. Dobson. 2004. "Postpartum Uterine Health in Cattle." Anim. Reprod. Sci. 82-83: 295-306. https://doi.org/10.1016/j.anireprosci.2004.04.006
Sheldon, I.M., A.N. Rycroft, B. Dogan, M. Craven, J.J. Bromfield, A. Chandler, M.H. Roberts, S.B. Price, R.O. Gilbert, and K.W. Simpson. 2010. "Specific Strains of Escherichia Coli Are Pathogenic for the Endometrium of Cattle and Cause Pelvic Inflammatory Disease in Cattle and Mice." PLoS One 5(2): e9192. https://doi.org/10.1371/journal.pone.0009192
Silva, E., M. Gaivão, S. Leitão, B.H. Jost, C. Carneiro, C.L. Vilela, L. Lopes da Costa, and L. Mateus. 2008. "Genomic Characterization of Arcanobacterium pyogenes Isolates Recovered from the Uterus of Dairy Cows with Normal Puerperium or Clinical Metritis." Vet Microbiol. 132: 111-8. https://doi.org/10.1016/j.vetmic.2008.04.033
Williams, E.J., D.P. Fischer, D.U. Pfeiffer, G.C. England, D.E. Noakes, H. Dobson, and I.M. Sheldon. 2005. "Clinical Evaluation of Postpartum Vaginal Mucus Reflects Uterine Bacterial Infection and the Immune Response in Cattle." Theriogenology. 63: 102-17. https://doi.org/10.1016/j.theriogenology.2004.03.017
Williams, E.J., D.P. Fischer, D.E. Noakes, G.C. England, A. Rycroft, H. Dobson, and I.M. Sheldon. 2007. "The Relationship between Uterine Pathogen Growth Density and Ovarian Function in the Postpartum Dairy Cow." Theriogenology 68: 549-59. https://doi.org/10.1016/j.theriogenology.2007.04.056
Williams, E.J., K. Sibley, A.N. Miller, E.A. Lane, J. Fishwick, D.M. Nash, S. Herath, G.C. England, H. Dobson, and I.M. Sheldon. 2008. "The Effect of Escherichia Coli Lipopolysaccharide and Tumour Necrosis Factor Alpha on Ovarian Function." Am. J. Reprod. Immunol. 60: 462-73. https://doi.org/10.1111/j.1600-0897.2008.00645.x