Onsite Sewage Treatment and Disposal Systems: Bacteria and Protozoa
Child drinking water from a hose.
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How to Cite

Lusk, Mary, Gurpal S. Toor, and Tom Obreza. 2011. “Onsite Sewage Treatment and Disposal Systems: Bacteria and Protozoa: SL350/SS552, 7/2011”. EDIS 2011 (8). Gainesville, FL. https://doi.org/10.32473/edis-ss552-2011.


Keeping disease-causing microorganisms out of groundwater used for drinking water supplies is important to protect human health. This 7-page fact sheet reports the sources of bacteria and protozoa in wastewater, discusses diseases associated with drinking water contaminated with wastewater, and then details their fate in septic systems. Written by Mary Lusk, Gurpal S. Toor, and Tom Obreza, and published by the UF Department of Soil and Water Science, July 2011. (Photo by Tara Piasio)

SL350/SS552: Onsite Sewage Treatment and Disposal Systems: Bacteria and Protozoa (ufl.edu)

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Arnade, L. "Seasonal Correlation of Well Contamination and Septic Tank Distance." Ground Water 37, no. 6 (1999):920-3. https://doi.org/10.1111/j.1745-6584.1999.tb01191.x

Bloetscher, F., and W.R. VanCott. "Impact of Septic Tanks on Wellhead Protection Efforts." Florida Water Resources Journal 51, no. 2 (1999):38-41.

Borchardt, M.A., P. Chyou, E.O. DeVries, and E.A. Belongia. "Septic System Density and Infectious Diarrhea in a Defined Population of Children." Environmental Health Perspectives 11, no. 5 (2003):742-8. https://doi.org/10.1289/ehp.5914

Bradford, S.A., Y.F. Tadassa, and Y. Pachepsky. "Transport of Giardia and Manure Suspensions in Saturated Porous Media." Journal of Environmental Quality 35, no. 3 (2006):749-57. https://doi.org/10.2134/jeq2005.0226

Darnault, C.J., P. Garnier, Y.J. Kim, K.L. Oveson, T.S. Steenhuis, J.Y. Parlange, M. Jenkins, W.C. Ghiorse, and P. Baveye. "Preferential Transport of Cryptosporidium parvum Oocysts in Variably Saturated Subsurface Environments." Water Environ Res 75, no. 2 (2003):113-20. https://doi.org/10.2175/106143003X140890

DeBorde, D.C., W.W. Woessner, B. Lauerman, and P.N. Ball. "Virus Occurrence and Transport in a School Septic System and Unconfined Aquifer." Ground Water 36, no. 5 (1998):825-34. https://doi.org/10.1111/j.1745-6584.1998.tb02201.x

Deng, M.Y., and D.O. Cliver. "Mixed Waste Studies with Viruses and Giardia." Proceedings of the Seventh National Symposium on Individual and Small Community Sewage Systems, ASAE, St. Joseph, MI, 1995.

Dillon, K.S., D.R. Corbett, J.P. Chanton, W.C. Burnett, and D.J. Furbish. "The Use of Sulfur Hexafluoride as a Tracer of Septic Tank Effluent in the Florida Keys." Journal of Hydrology 220 (1999):129-40. https://doi.org/10.1016/S0022-1694(99)00073-6

Feachem, R.G., D.J. Bradley, H. Garelick, and D.D. Mara. Health Aspects of Excreta and Sullage Management - A State-of-the-Art Review. Washington, D.C.: The World Bank, 1980.

Fong, T., L.S. Mansfield, D.L. Wilson, D.J. Schwab, S.L. Molloy, and J.B. Rose. "Massive Microbiological Groundwater Contamination Associated with a Waterborne Outbreak in Lake Erie, South Bass Island, Ohio." Environmental Health Perspectives 115, no. 6 (2007):856-64.

Gerba, C.P., and G. Britton. "Microbial Pollutants: Their Survival and Transport Patterns to Groundwater." In Groundwater Pollution Microbiology, G. Britton and C. Gerba (eds.). New York: John Wiley and Sons, 1984.

Gerba, C.P., C. Wallis, and J.L. Melnick. "Fate of Wastewater Bacteria and Viruses in Soil." Journal of Irrigation and Drainage 101 (1975):157-75.

Hagedorn, C., E.L. McCoy, and T.M. Rahe. "The Potential for Ground Water Contamination from Septic Effluents." Journal of Environmental Quality 10 (1981):1-8. https://doi.org/10.2134/jeq1981.00472425001000010001x

Horan, N.J. "Faecal Indicator Organisms." In The Handbook of Water and Wastewater Microbiology D. Mara and N.J. Horan (eds.), 104-12. San Diego, CA: Academic Press, 2003.

Katz, B.G., S.M. Eberts, and L.J. Kauffman. "Using Cl/Br ratios and Other Indicators to Assess Potential Impacts on Groundwater Quality from Septic Systems: A Review and Examples from Principle Aquifers in the United States." Journal of Hydrology 397 (2010):151-66. https://doi.org/10.1016/j.jhydrol.2010.11.017

Levett, K.J., J.L. Vanderzalm, D.W. Page, and P.J. Dillon. "Factors Affecting the Performance and Risks to Human Health of On-site Wastewater Treatment Systems." Water Science and Technology 62 (2010):1499-509. https://doi.org/10.2166/wst.2010.434

Logan, A.J., T.K. Stevik, R.L. Siegrist, and R.M. Ronn. "Transport and Fate of Cryptosporidium parvum in Intermittent Sand Filters during Wastewater Treatment." Proceedings of the Ninth National Symposium on Individual and Small Community Sewage Systems, ASAE, St. Joseph, MI, 2001.

Lowe, K.S., N.K. Rothe, J.M.B. Tomaras, K. DeJong, M.B. Tucholke, J.E. Drewes, J.E. McCray, and J. Munakata-Marr. "Influent Constituent Characteristics of the Modern Waste Stream from Single Sources: Literature Review." Water Environment Research Foundation. Technical Report 04-DEC-1a, 2007.

Mawdsley, J.L., R.D. Bardgett, R.J. Merry, B.F. Pain, and M.K. Theodorou. "Pathogens in Livestock Waste, Their Potential for Movement through Soil and Environmental Pollution." Applied Soil Ecology 2 (1995):1-15.

McCray, J., K.S. Lowe, M. Geza, J. Drewes, S. Roberts, A. Wunsch, D. Radcliffe, J. Amador, J. Atoyan, T. Boving, D. Kalen, and G. Loomis. "State of the Science: Review of Quantitative Tools to Determine Wastewater Soil Treatment Unit Performance." Water Environment Research Foundation. Technical Report DEC1R06, 2009. https://doi.org/10.2166/9781780403564

Meeroff, D.E., F. Bloetscher, T. Bocca, and F. Morin. "Evaluation of Water Quality Impacts of Onsite Treatment and Disposal Systems on Urban Coastal Waters." Water Air Soil Pollution 192 (2008):11-24. https://doi.org/10.1007/s11270-008-9630-2

Moelants, N., G. Jannsen, I. Smets, and J. VanImpe. "Field Performance Assessment of Onsite Individual Wastewater Treatment Systems." Water Science and Technology 58 (2008):1-6. https://doi.org/10.2166/wst.2008.325

Ozkan, S., H. Tuzun, N. Gorer, M. Ceyhan, S. Aycan, S. Albayrak, and M.A. Bumin. "Water Usage Habits and the Incidence of Diarrhea in Rural Ankara, Turkey." Transactions of the Royal Society of Tropical Medicine and Hygiene 101, no.1 (2007):1131-5. https://doi.org/10.1016/j.trstmh.2007.05.011

Roeder, E., and W. Brookman. "Performance of Aerobic Treatment Units: Monitoring Results from the Florida Keys." Journal of Environmental Health 69, no.4 (2006):17-22.

Sjogren, R.E. "Prolonged Survival of an Environmental Escherichia coil in Laboratory Soil Microcosms." Water Air Soil Pollution 75 (1994):389-403. https://doi.org/10.1007/BF00482948

Snowden, J.A., D.O. Clover, and J.C. Converse. "Human and Animal Wastes Mixed for Disposal to Land: Inactivation of Viruses and Parasites in a Laboratory Model." Proceedings of the Fifth National Symposium on Individual and Small Community Sewage Systems, ASAE, St. Joseph, MI, 1989.

Stevik, T.K., K. Aa, G. Ausland, and J.F. Hanssen. "Retention and Removal of Pathogenic Bacteria in Wastewater Percolating through Porous Media: A Review." Water Research 38 (2004):1355-67. https://doi.org/10.1016/j.watres.2003.12.024

Toor, G.S., M. Lusk, and T. Obreza. "Onsite Sewage Treatment and Disposal Systems: An Overview." University of Florida - IFAS, Gainesville, FL. Accessed July 8, 2011. http://edis.ifas.ufl.edu/ss549.

U.S. EPA. 2011. A Homeowner's Guide to Septic Systems. Accessed July 8, 2011. http://www.epa.gov/owm/septic/pubs/homeowner_guide_long.pdf.

Weiss, T.H., A.L. Mills, G.M. Hornberger, and J.S. Herman. "Effect of Bacterial Shape on Transport of Bacteria in Porous Media." Environmental Science and Technology 29 (1995):1737-40. https://doi.org/10.1021/es00007a007

Weissman, J.B., G.F. Craun, D.N. Lawrence, R.A. Pollard, M.S. Saslaw, and E.J. Gangarosa. "An Epidemic of Gastroenteritis Traced to a Contaminated Public Water Supply." American Journal of Epidemiology 103 (1976):391. https://doi.org/10.1093/oxfordjournals.aje.a112238

Yates, M.V. "Septic Tank Density and Groundwater Contamination." Ground Water 23, no. 5 (2006):586-91. https://doi.org/10.1111/j.1745-6584.1985.tb01506.x