Laboratory Experiment on Gene Subcloning for ChE Students
A laboratory experiment for senior chemical engineering students is described. Students are introduced to basic techniques of molecular biology by performing a subcloning experiment. The experiment requires five laboratory sessions and students are exposed to polymerase chain reaction, plasmid purification, DNA ligation, bacterial transformation, and protein expression. Hands-on experience with these techniques can provide both appreciation for and knowledge of the techniques and processes involved with DNA cloning that a lecture course alone cannot provide.