Illustrating Chromatography With Colorful Proteins


  • Brian G. Lefebvre Rowan University
  • Stephanie Farrell Rowan University
  • Richard S. Dominiak Rowan University


Advances in biology are prompting new discoveries in the biotechnology, pharmaceutical, medical technology, and chemical industries. This paper presents a detailed description of an anion exchange chromatography experiment using a pair of colorful proteins and summarizes the effect of operating parameters on protein separation. This experiment could be applied in three settings: core chemical engineering courses focused on separation processes, unit operation laboratory courses, and elective courses focused on biochemical engineering or bioseparations.

Author Biographies

Brian G. Lefebvre, Rowan University

Brian G. Lefebvre is an assistant professor of chemical engineering at Rowan University. He received his B.Ch.E. from the University of Minnesota in 1997 and his Ph.D. from the University of Delaware in 2002. Prior to joining Rowan, he performed postdoctoral research in protein structural biology at the University of Pennsylvania. His primary teaching interest is integrating biochemical and biomolecular engineering in the engineering curriculum.

Stephanie Farrell, Rowan University

Stephanie Farrell is an associate professor of chemical engineering at Rowan University. She received her B.S. from the University of Pennsylvania, her M.S. from Stevens Institute of Technology, and her Ph.D. from the New Jersey Institute of Technology. She has been recognized for her impact on chemical engineering education with the 2006 Robert G. Quinn Award, the 2004 ASEE National Outstanding Teaching Medallion, and the 2002 ASEE Ray W. Fahien Award.

Richard S. Dominiak, Rowan University

Richard S. Dominiak is currently employed at Foster Wheeler. He received his B.S. in chemical engineering from Rowan University in 2006. While at Rowan University he spent two years as a research assistant on this topic.