Other Contributions: A New Method for the Differential Staining of Internal Organs of Nematodes

Abstract

The use of a .05% (w/v) toluidine blue solution in 0.05M, pH 4.60 phosphate buffer for in toto staining of nematodes was studied with Hoplolaimus galeatus and Helicotylenchus dihystera. Treatment of fixed specimens in the solution for 7 hrs at 60C resulted in general staining. When stained specimens were immersed for 5 hrs at 50-60C in phosphate buffer (0.01M, pH - 4.6) good differentiations of the esophagous and reproductive structures was obtained.