Microarray Analysis of Gene Expression in Soybean Roots Susceptible to the Soybean Cyst Nematode Two Days Post Invasion

  • R. Khan
  • N. Alkharouf
  • H. Beard
  • M. MacDonald
  • I. Chouikha
  • S. Meyer
  • J. Grefenstette
  • H. Knap
  • B. Matthews
Keywords: glycine max, heterodera glycines, microarray, plant-pathogen interaction, sgmd, signaling pathway, soybean cyst nematode, soybean genomics microarray database, syncytium

Abstract

Soybean root cells undergo dramatic morphological and biochemical changes during the establishment of a feeding site in a compatible interaction with the soybean cyst nematode (SCN). We constructed a cDNA microarray with approximately 1,300 cDNA inserts targeted to identify differentially expressed genes during the compatible interaction of SCN with soybean roots 2 days after infection. Three independent biological replicates were grown and inoculated with SCN, and 2 days later RNA was extracted for hybridization to microarrays and compared to noninoculated controls. Statistical analysis indicated that approximately 8% of the genes monitored were induced and more than 50% of these were genes of unknown function. Notable genes that were more highly expressed 2 days after inoculation with SCN as compared to noninoculated roots included the repetitive proline-rich glycoprotein, the stress-induced gene SAM22, ß-1,3-endoglucanase, peroxidase, and those involved in carbohydrate metabolism, plant defense, and signaling.
Published
2004-09-15
Section
Articles