Fungal Parasites of the Potato Cyst Nematode Globodera rostochiensis: Isolation and Reinfection

  • Carin Dackman


Fungal parasitism of eggs of the potato cyst nematode Globodera rostochiensis was 1, 3, and 17% at three sites in Sweden. The fungi isolated most frequently from infected eggs were a Septocylindrium-like fungus ( 19 %), Exophiala spp. (17 %), and Cylindrocarpon spp. (13 %). Verticillium suchtasporium was isolated from infected eggs at a low frequency (4%). In laboratory experiments V. suchlasporium infected 93% of the eggs within cysts after 10 days on dilute corn meal agar. This species showed chitinase and protease activity. Infection of eggs by the Septocylindrium-like fungus was moderate, whereas Cylindrocarpon destructans and Cladosporium cladosporoides did not infect eggs. No chitinase activity was found in these fungi, but protease activity was recorded in all. Growth of the fungi in cysts did not influence the number of physiologically disordered eggs. Key words: egg parasite, infection, chitinase, physiological disorder, Globodera rostochiensis, Verticillium suchlasporium, Cylindrocarpon destructans.