DNA Restriction Fragment Length Polymorphism in Races of the Soybean Cyst Nematode, Heterodera glycines

Authors

  • A. Kalinski
  • R. N. Huettel

Abstract

Restriction endonuclease digests of total DNA from races 3, 4, and 5 of the soybean cyst nematode, Heterodera glycines, have been analyzed on agarose gels. DNA fragment patterns of race 4 were completely different from those patterns obtained for races 3 and 5 by all eight restriction enzymes tested. Differences in long and short restriction DNA fragments generated by the enzyme Msp I or its isoschizomer, Hpa II, were detected between race 3 and 5 digestion profiles. Rapid DNA isolation followed by its digestion with either Msp I or Hpa II enzymes and visualization of repetitive DNA fragments in agarose gels provided a diagnostic assay for the populations of the three races examined in this study. Key words: DNA electrophoresis, genetic divergence, Glycine max, Heterodera glycines, nematode genome, restriction fragment, soybean, soybean cyst nematode.

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Published

1988-10-15

Issue

Vol. 20, No. 4 (October 1988)

Section

Articles

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