A Method for Isolation of <I>Pasteuria penetrans</I> Endospores for Bioassay and Genomic Studies
Keywords:
endospores, method, Pasteuria penetrans.Abstract
A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.Downloads
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