A Method for Isolation of <I>Pasteuria penetrans</I> Endospores for Bioassay and Genomic Studies

  • Jenora T. Waterman
  • David McK. Bird
  • Charles H. Opperman
Keywords: endospores, method, Pasteuria penetrans.

Abstract

A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.
Published
2006-03-15
Section
Articles