Control of Soybean Cyst Nematode by Chitinolytic Bacteria with Chitin Substrate

  • Honglin Tian
  • Robert D. Riggs
  • Devany L. Crippen
Keywords: biological control, chitin, chitinase-producing bacteria, chitinolytic bacteria, glycine max, heterodera glycines, soybean, soybean cyst nematode


Sixty-four chitinolytic bacterial isolates from soybean fields in Arkansas were tested for suppression of soybean cyst nematode (Heterodera glycines) in a heat-treated silt loam soil amended with 0.6% (w/w) chitin in a greenhouse. Five isolates consistently reduced numbers of H. glycines compared to controls receiving neither chitin nor bacteria, or only chitin. Four of the five isolates interacted with the chitin substrate to enhance its effectiveness in reducing numbers of the nematode. The size of the clear-zone produced by some of the isolates in colloidal chitin medium, an indication of chitinolytic activity in vitro, was not related to suppression of nematode numbers in soil.