Quantification of Endospore Concentrations of Pasteuria penetrans in Tomato Root Material

Authors

  • Z. X. Chen
  • D. W. Dickson
  • T. E. Hewlett

Abstract

Six methods for quantification of the endospore concentrations of Pasteuria penetrans from tomato roots are described. Mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. These methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and enzymatic disruption + flotation (25.8 million) methods. A centrifugation bioassay method gave the lowest estimation of 12.7 million. Key words: bacterium, biological control, endospore, Lycopersicon esculentum, Meloidogyne arenaria, method, Pasteuria penetrans, root-knot nematode, tomato.

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Published

1996-03-15

Issue

Vol. 28, No. 1 (March 1996)

Section

Articles

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