Detection of Intraspecific Diversity of Heterodera glycines Using Isozyme Phenotypes
Abstract
Twelve populations of Heterodera glycines from the United States (8), China (2), Japan (1), and Colombia (1) were surveyed for phenotypic intraspecific variability in 42 enzyme systems. Activity of 20 enzymes was detected following isoelectric focusing in polyacrylamide gels of extracts from mass homogenates and single females. Five enzymes, aspartate aminotransferase, phosphoglucose isomerase,[alpha]- and[beta]-esterases, and hexokinase were the most useful for detecting intraspecific variability. Phenotypic variability between single females was best demonstrated with[alpha]- and[beta]-esterases and acid phosphatase enzyme systems. These results suggest that isoelectric focusing in conjunction with sensitive enzyme systems can be used to detect phenotypic variation between individual nematodes from the same population. The unusual phenotypic variability detected in the H. glycines population from Virginia indicates that the genetic diversity of this population is complex. Key words: enzyme, genetic diversity, Heterodera glycines, intraspecific variability, isoelectric focusing, isozyme, race.Downloads
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