Morphological Comparison of Second-Stage Juveniles of Six Populations of Meloidogyne hapla by SEM

Abstract

External morphology of second-stage juveniles of six populations of Meloidogyne hapla, hclonging to two cytological races (A and B), and one population each of M. arenaria, M, incognita, and M. javanica was compared by scanning electron microscopy (SEM). Race A of M. hapla included three facuhatively parthenogenetic populations with haploid chromosome numbers of 15. 16, and 17; race B consisted of three mitotically parthenogenetic populations with somalic chromosome numhers of 45, 45, and 48. The mitotically parthenogenetic populations of M. arenaria, M. incognita, and M. javanica had 54, 41-43, and 44 chromosomes, respectively. Observations were made on head structures, lateral field, excretory pore, anal opening, and tail. Head morphology, including shape and proportion of labial disc and lips, expression of labial and cephalic sensilla, and markings on head region, was distinctly different for each species. M. Hapla populations of race A were distinct from each other but showed much intrapopulatiou variation in head morphology. Populations of race B were different from those of race A and were very stable and quite similar in head morphology. Considerable inter- and intrapopulatiou variation made the structure of the lateral field, excretory pore, anal opening, and tail of little value in distinguishing species or populations. The results are discussed in relation to earlier SEM observations on the genus Helerodera. Key Words: M. arenaria, M. incognita, M. javanica.