Mass Culturing of Ditylenchus dipsaci to Yield Large Quantities of Inoculum

  • L. R. Faulkner
  • D. B. Bower
  • D. W. Evans
  • J. H. Elgin, Jr.

Abstract

Methods are described for rearing large quantities of Ditylenchus dipsaci on alfalfa tissues. Nematodes and alfalfa seed were disinfected and nematodes were reared in quantities sufficient to provide a continuous supply of inoculum for our alfalfa-breeding program. Nematodes reproduced best in darkness at 20-25 C. Cultures reached maximum numbers in 3-6 wk. Key Words: tissue culture, alfalfa, stem nematode, Medicago sativa.
Published
1974-07-15
Section
Articles