A Comparison of Preparation Techniques in Taxonomic Studies of Longidorus africanus Merny
Abstract
A comparison was made of ten different techniques for killing, fixing, and mounting Longidorus a[ricanus Merny for microscopic study. The most satisfactory specimens were those killed by Seinhorst's method, fixed in FAA and mounted in glycerin by the slow method. Specimens killed by "gentle heat," fixed in FAA and mounted in glycerin were also acceptable as were those killed by hot formalin and mounted in glycerin or processed by Baker's method. Less satisfactory were: nematodes killed by "gentle heat," fixed in formalin and mounted in glycerin and specimens killed by vapor phase perfusion or by Hopper's lethal stain, both latter groups were mounted in glycerin after fixation in formalin. Killing with cold formalin, gradual heat (60 C for 15 min), or by storage in distilled water produced poorly defined specimens. Nematodes killed by hot formalin, and processed to glycerin by the slow method, maintained their live dimensions. Reduction in length occurred in specimens killed by cold formalin, by storage, or treated with solutions containing acetic or propionic acids. Nematodes processed by Baker's method increased in size. Other minor modifications occurred in specimens processed by the different methods. Esophageal definition was best in nematodes killed with formalin, hot or cold. There is no correlation between position of the posterior part of the esophagus and position of the onchiostyle.Downloads
Published
Issue
Section
License
Copyright and Permissions
All material published by the Society of Nematologists (SON), except for papers prepared by United States and Canadian government employees, is copyrighted and protected under the U.S. copyright law. Under the Copyright Act of 1976, the term of copyright for materials registered by an organization is 75 years from the date first published. Before publishing any manuscript, SON requires that authors transfer full and complete ownership of any copyright to SON by signing a JON Page Charge/Copyright Form (.pdf). SON then registers the copyright. Subsequent use of published materials requires written permission from the SON and may be obtained by contacting the current Editor-in-Chief and state where and how the material will be used.
The author warrants that the article is an original work not published elsewhere in whole or in part, except in abstract form, and that the author has full power to make this grant. If portions of the article have been published previously, then the author warrants that permission has been obtained from the copyright holder and the author will submit a copy of the permission release with this copyright transfer form.
SON shall claim no proprietary right other than copyright. Authors and coauthors retain the right to revise, adapt, modify, or otherwise use all or part of the article in future works of the author(s), such as press releases, lectures, and reviews, provided that all such use is for the personal noncommercial benefit of the author(s). All patent rights are retained by the author(s).
