Diagnostic characters within ITS2 DNA support molecular identification of Anastrepha suspensa (Diptera: Tephritidae)

Authors

  • Norman Barr USDA APHIS PPQ Center for Plant Health Science and Technology Mission Laboratory, Edinburg, TX 78541, USA
  • Raul Ruiz-Arce USDA APHIS PPQ Center for Plant Health Science and Technology Mission Laboratory, Edinburg, TX 78541, USA
  • Oscar Obregón USDA APHIS PPQ Center for Plant Health Science and Technology Mission Laboratory, Edinburg, TX 78541, USA
  • Robert Shatters USDA ARS Horticultural Research Laboratory, Fort Pierce, FL 34945, USA
  • Allen L. Norrbom USDA ARS Systematic Entomology Laboratory, Washington, DC 20013, USA
  • Norma Nolazco Servicio Nacional de Sanidad Agraria, Lima 12, Peru
  • Donald Thomas USDA ARS Cattle Fever Tick Research Laboratory. Edinburg, TX 78541, USA

Keywords:

fruit fly, Anastrepha ludens, Anastrepha obliqua, concerted evolution

Abstract

Summary

An approximately 220 bp fragment of the internal transcribed spacer 2 (ITS2) was screened as a diagnostic trait of Anastrepha suspensa (Loew) (Diptera: Tephritidae) and other pest fruit fly species in the genus Anastrepha. The majority (96%) of the sites in this fragment were invariant among the test species, but A. suspensa can be separated from other species by using 2 diagnostic characters. Similarly, A. ludens (Loew) and A. obliqua (Macquart) can be distinguished from other species based on 1 fixed character each. There is evidence of intraspecific ITS2 variation in 5 species tested, consistent with species complexes and incomplete homogenization through the process of concerted evolution.

 

Sumario

Se examinó un fragmento de aproximadamente 220 pb del espaciador transcrito interno 2 (ITS2) como diagnóstico de Anastrepha suspensa (Loew) (Diptera: Tephritidae) y otras moscas de plagas en el género Anastrepha. La mayoría (96%) de los sitios en este fragmento fueron invariantes entre las especies de prueba, pero se puede separar A. suspensa de otras especies usando 2 caracteres diagnósticos. Similarmente, se pueden distinguir A. ludens (Loew) y A. obliqua (Macquart) de otras especies basadas en 1 carácter fijo cada una. Hay evidencia de variación intraespecífica ITS2 en 5 especies probadas, consistentes con complejos de especies y homogeneización incompleta a través del proceso de evolución concertada. 

 

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Author Biography

Norman Barr, USDA APHIS PPQ Center for Plant Health Science and Technology Mission Laboratory, Edinburg, TX 78541, USA

PPQ Mission Laboratory

Assistant Laboratory Director

Downloads

Published

2017-04-16

Issue

Vol. 100, No. 1 (March 2017)

Section

Scientific Notes

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