Diagnostic characters within ITS2 DNA support molecular identification of Anastrepha suspensa (Diptera: Tephritidae)
Keywords:
fruit fly, Anastrepha ludens, Anastrepha obliqua, concerted evolutionAbstract
Summary
An approximately 220 bp fragment of the internal transcribed spacer 2 (ITS2) was screened as a diagnostic trait of Anastrepha suspensa (Loew) (Diptera: Tephritidae) and other pest fruit fly species in the genus Anastrepha. The majority (96%) of the sites in this fragment were invariant among the test species, but A. suspensa can be separated from other species by using 2 diagnostic characters. Similarly, A. ludens (Loew) and A. obliqua (Macquart) can be distinguished from other species based on 1 fixed character each. There is evidence of intraspecific ITS2 variation in 5 species tested, consistent with species complexes and incomplete homogenization through the process of concerted evolution.
Sumario
Se examinó un fragmento de aproximadamente 220 pb del espaciador transcrito interno 2 (ITS2) como diagnóstico de Anastrepha suspensa (Loew) (Diptera: Tephritidae) y otras moscas de plagas en el género Anastrepha. La mayoría (96%) de los sitios en este fragmento fueron invariantes entre las especies de prueba, pero se puede separar A. suspensa de otras especies usando 2 caracteres diagnósticos. Similarmente, se pueden distinguir A. ludens (Loew) y A. obliqua (Macquart) de otras especies basadas en 1 carácter fijo cada una. Hay evidencia de variación intraespecífica ITS2 en 5 especies probadas, consistentes con complejos de especies y homogeneización incompleta a través del proceso de evolución concertada.
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