Comparative transcriptome analysis of three Bactrocera dorsalis (Diptera: Tephritidae) organs to identify functional genes in the male accessory glands and ejaculatory duct

Authors

  • Chuan-Bei Tian Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
  • Dong Wei Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
  • Lin-Fan Xiao Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
  • Wei Dou Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
  • Huai Liu Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
  • Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China

Keywords:

oriental fruit fly, comparative transcriptome, accessory gland protein, seminal fluid protein, reproduction

Abstract

The insect male accessory glands/ejaculatory duct (MAG/ED) are important tissues of the male reproductive system. The MAG/ED’s functions in reproduction have been well studied in Drosophila (Diptera: Drosophilidae) but remain largely unknown in the important agricultural pest Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). In the present study, we re-assembled the transcriptome datasets of B. dorsalis’s fat body, testis, and MAG/ED and compared these tissue-specific transcriptomes. Clean reads from these transcriptome data sets were de novo re-assembled and clustered into 31,782 unigenes (average 922 bp). In total, 21,306 unigenes were functionally annotated by Blasting against online databases. Comparative transcriptomic analysis identified numerous genes that were identified with the expressed tissue-bias patterns. Some MAG/ED-specific genes potentially involved in spermatozoa motility and capacitation (e.g., perlucin, glucose dehydrogenase, lipase), mating regulation (pheromone-binding protein–related protein), and immunity (lectin) were identified in B. dorsalis. The expressions of some of these genes were further validated by realtime quantitative polymerase chain reaction at transcriptional level. All of these identifications will help us to explore the physiological regulation of mating and reproduction in B. dorsalis in the future.

Resumen
Las glándulas accesorias masculinas/conducto eyaculador (GAM/CE) de los insectos son tejidos importantes del sistema reproductivo masculino. Las funciones de GAM/CE en la reproducción han sido bien estudiadas en Drosophila (Diptera: Drosophilidae), pero siguen siendo poco conocidas en la importante plaga agrícola Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). En el presente estudio, re-ensamblamos los datos de transcriptoma de la grasa del cuerpo, los testículos y los GAM/CE de B. dorsalis y comparamos estos transcriptomas específicos de tejidos. Las lecturas claras de estos conjuntos de datos de transcriptoma se volvieron a ensamblar de nuevo y se agruparon en 31.782 unigenes (promedio de 922 bp). En total, 21.306 unigenes fueron anotados funcionalmente por Blasting contra bases de datos en línea. El análisis transcriptómico comparativo identificó numerosos genes que se identificaron con los patrones de sesgo de tejido expresado. En B. dorsalis se identificaron algunos genes especıficos de GAM/CE potencialmente implicados en motilidad y capacitación de espermatozoides (por ejemplo, perlucina, deshidrogenasa de glucosa, lipasa), regulación de apareamiento (proteına relacionada con proteına de unión a feromonas) e inmunidad (lectina). Las expresiones de algunos de estos genes fueron aún más validados por la reacción en cadena de la polimerasa cuantitativa en tiempo real a nivel transcripcional. Todas estas identificaciones nos ayudarán a explorar la regulación fisiológica del apareamiento y la reproducción en B. dorsalis en el futuro.

 

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Author Biography

Jin-Jun Wang, Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China

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Published

2017-04-16

Issue

Vol. 100, No. 1 (March 2017)

Section

Research Papers

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