Effects of a pathogenic Beauveria bassiana (Hypocreales: Cordycipitaceae) strain on detoxifying and protective enzyme activities in Xylotrechus rusticus (Coleoptera: Cerambycidae) larvae

Authors

  • Jun-Nan Ding Institute of Corp Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, People’s Republic of China College of Forestry, Northeast Forestry University, Harbin 150040, People’s Republic of China
  • Hui-Hui Zhang College of Resources and Environment, Northeast Agricultural University, Harbin 150030, People’s Republic of China
  • De-Fu Chi College of Forestry, Northeast Forestry University, Harbin 150040, People’s Republic of China

Keywords:

forest pest, detoxifying enzyme, protective enzyme, quarantine pest, high virulence

Abstract

In recent years, biological methods have been used widely for controlling stem-boring pests in forests. In this study, the larvae of Xylotrechus rusticus (L.) (Coleoptera: Cerambycidae) were infected with 4 strains of 2 species of Beauveria (Hypocreales: Cordycipitaceae). Larval detoxifying and protective enzyme activities were measured at different times after infection. The results showed significant differences in the pathogenicity of the 4 strains. The most virulent strain, Beauveria bassiana (Bals.-Criv) Vuill. strain BbCC01, caused 93.3% mortality, with an estimated LT50 of 4.69 d. The detoxifying and protective enzyme activities in larvae infected with strain BbCC01 changed significantly. To resist the infection, the activities of the detoxifying enzymes (carboxylesterase, glutathione S-transferase, and acetylcholinesterase) first increased and then slowly decreased. The activities of the protective enzymes (catalase, superoxide dismutase, and peroxidase) showed a similar trend. The enzyme activities peaked at 72 to 96 h post-infection. In this study, the B. bassiana strain BbCC01 proved to exhibit high virulence towards X. rusticus larvae. This research provides important information regarding the biological control of X. rusticus and elucidates the patterns of detoxifying and protective enzyme activities in the susceptible larvae.

 

Resumen

En los últimos años, los métodos biológicos se han utilizado ampliamente para controlar plagas barrenadoras de tallos en bosques. En este estudio, las larvas de Xylotrechus rusticus (L.) (Coleoptera: Cerambycidae) fueron infectadas con 4 cepas de 2 especies de Beauveria (Hypocreales: Cordycipitaceae). Se midieron la actividad de enzimas de desintoxificación y de protección en las larvas en diferentes momentos después de la infección. Los resultados mostraron diferencias significativas en la patogenicidad de las 4 cepas. La cepa más virulenta, Beauveria bassiana (Bals.-Criv) Vuill. cepa BbCC01, causó 93.3% de mortalidad, con un estimado TL50 de 4.69 d. Las actividades de desintoxicación y de protección de las enzimas en las larvas infectadas con la cepa BbCC01 cambiaron significativamente. Para resistir a la infección, las actividades de las enzimas detoxificantes (carboxilesterasa, glutatión S-transferasa, y la acetilcolinesterasa) primero aumentaron y luego disminuyeron lentamente. Las actividades de las enzimas protectoras (catalasa, superóxido dismutasa, y peroxidasa) mostraron una tendencia similar. Las actividades enzimáticas alcanzaron un máximo de 72 a 96 horas después de la infección. En este estudio, la cepa B. bassiana BbCC01 resultó exhibir una alta virulencia hacia larvas de X. rusticus. Esta investigación provee información importante sobre el control biológico de X. rusticus y aclara los patrones de actividad de las enzimas desintoxicantes y de protección en las larvas susceptibles.

 

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Published

2015-09-30

Issue

Vol. 98, No. 4 (December 2015)

Section

Research Papers

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