Molecular diagnostic technique for the differentiation of the Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae) from other subterranean termites by multiplex-PCR

Authors

  • Mark A. Janowiecki Department of Entomology, University of Arkansas, Fayetteville, Arkansas, USA
  • Allen L. Szalanski Department of Entomology, University of Arkansas, Fayetteville, Arkansas, USA

Keywords:

Coptotermes formosanus, molecular genetics, invasive termite, subterranean termite

Abstract

The Formosan subterranean termite Coptotermes formosanus Shriaki; (Isoptera: Rhinotermitidae), is a major pest that is spreading throughout the southeastern United States. Morphological identification of worker specimens is not possible using available taxonomic keys based on morphological traits. A multiplex PCR protocol was developed that can differentiate the Formosan subterranean termite from other termite species in a single PCR reaction. This multiplex PCR protocol simplifies previous molecular diagnostic techniques.

 

Sumario

La termita subterránea de Formosa, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), es una plaga importante que se está extendiendo desde el sureste de los Estados Unidos. La identificación morfológica de los especimenes de trabajadores no es posible usando las claves basadas en características morfológicas. Un protocolo de PCR múltiple fue desarrollado que puede diferenciar la termita subterránea de Formosa en una reacción de PCR. Este protocolo PCR multiplex reemplaza las técnicas diagnósticas moleculares anteriores, que requieren múltiples reacciones de PCR.

 

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Issue

Vol. 98, No. 1 (March 2015)

Section

Scientific Notes

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