Determination of Number of Instars of Rhyssomatus subtilis (Coleoptera: Curculionidae) Based on Head Capsule Widths
Keywords:
black soybean weevil, Hcap program, Dyar’s rule, pest, life cycleAbstract
Rhyssomatus subtilis Fiedler (Coleoptera: Curculionidae) is an important pest of the soybean crop in northwestern Argentina. Few studies have been made on specific parameters of its life history and ecology. The aim of this study was to determine the number of larval stages of R. subtilis. One thousand and eighteen larvae were collected from soybean plants during 2 yr (2011 and 2013), and head capsule width of each larva was measured. For analysis of data, the Hcap program and Dyar’s rule were used. The Hcap program showed 4 different peaks in the frequency distribution of the head capsule widths. This result also agreed with Dyar’s rule that revealed a perfect geometric larval growth pattern for each instar by regression analysis. The excellent fit to a linear model, indicates that no instar was overlooked. This research identified 4 instars for R. subtilis.
Rhyssomatus subtilis Fiedler (Coleoptera: Curculionidae) es una importante plaga del cultivo de soja en el Noroeste Argentino. Existen pocos estudios acerca de parámetros específicos del ciclo de vida y de comportamiento ecológico. El objetivo de este estudio fue determinar el número de instares larvales de R. subtilis. En total, 1018 larvas fueron colectadas del cultivo de soja durante 2011 y 2013. Se midió el ancho de la cápsula cefálica de cada larva. Para el análisis de los datos se utilizó el programa Hcap y la regla de Dyar. El programa Hcap mostró cuatro picos distintos en la distribución de frecuencias de la anchura de la cápsula cefálica. Estos resultados concuerdan con la regla de Dyar que muestra un perfecto crecimiento geométricos de las larvas para cada instar representada por la línea de regresión. El excelente ajuste del modelo lineal, indica que no hay instares solapados. Esta investigación identificó cuatro instares larvales para R. subtilis.
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