Effects of Duration of Cold Storage of Host Eggs on Percent Parasitism and Adult Emergence of Each of Ten Trichogrammatidae (Hymenoptera) Species

Authors

  • Paulo Roberto de Carvalho Spínola-Filho Insetário G.W.G. de Moraes, Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, CP: 135, 39404-006, Montes Claros, Minas Gerais, Brazil
  • Germano Leão Demolin Leite Insetário G.W.G. de Moraes, Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, CP: 135, 39404-006, Montes Claros, Minas Gerais, Brazil
  • Marcus Alvarenga Soares Programa de Pós-Graduação em Produção Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 39100-000, Diamantina, Minas Gerais, Brazil
  • Anarelly Costa Alvarenga Insetário G.W.G. de Moraes, Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, CP: 135, 39404-006, Montes Claros, Minas Gerais, Brazil
  • Paula Daiana de Paulo Insetário G.W.G. de Moraes, Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, CP: 135, 39404-006, Montes Claros, Minas Gerais, Brazil
  • Leonardo David Tuffi-Santos Insetário G.W.G. de Moraes, Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, CP: 135, 39404-006, Montes Claros, Minas Gerais, Brazil
  • José Cola Zanuncio Departamento de Biologia Animal, Universidade Federal de Viçosa, 36571-000, Viçosa, Minas Gerais, Brazil

Keywords:

biological control, insects rearing, Lepidoptera, Trichogramma

Abstract

Improving parasitoid mass rearing techniques is important to reduce costs of biological control programs and supply natural enemies at times of high demand. The objective of this study was to evaluate the suitability of Anagasta kuehniella (Lepidoptera: Pyralidae) eggs stored at 5 °C for different time periods as a host for Trichogrammatoidea annulata (Hymenoptera: Trichogrammatidae) and for 9 Trichogramma species (T. acacioi, T. atopovirilia, T. benneti, T. brasiliensis, T. bruni, T. demoraesi, T. galloi, T. pretiosum, and T. soaresi). The experiment had a factorial design with 10 parasitoid species, 9 host storage periods (5, 10, 15, 20, 25, 30, 35 and 40 days, and a no storage control) and 20 replications, each consisting of one card (0.4 × 2.0 cm) with 40.70 ± 0.03 eggs of A. kuehniella. Trichogrammatoidea annulata, T. acacioi, T. brasiliensis, T. bruni, T. pretiosum, and T. soaresi parasitized eggs of A. kuehniella stored up to 24 days; T. atopovirilia parasitized eggs stored up to 16 days. Trichogramma demoraesi and T. benneti parasitized eggs stored for 15 days and T. galloi parasitized eggs stored for up to 13 days. The percentage of parasitized eggs decreased when the storage period increased. Among the tested parasitoids T. acacioi parasitized eggs stored for longer periods and showed the highest percentage both of parasitism and adult emergence.

 

Mejorar las técnicas de cría masiva es importante para reducir los costos de programas de control biológico y proveer enemigos naturales en periodos de alta demanda. El objetivo de este estudio fue evaluar la idoneidad de huevos de Anagasta kuehniella (Lepidoptera: Pyralidae), almacenados a 5 °C por varios periodos de tiempo, como hospederos de Trichogrammatoidea annulata (Hymenoptera: Trichogrammatidae) y nueve especies de Trichogramma (T. acacioi, T. atopovirilia, T. benneti, T. brasiliensis, T. bruni, T. demoraesi, T. galloi, T. pretiosum, and T. soaresi). El experimento tuvo un diseño factorial con 10 especies de parasitoides, 9 periodos de almacenamiento (5, 10, 15, 20, 25, 30, 35 y 40 días; y no almacenamiento como control) y 20 réplicas, cada una consintiendo en un pedazo de cartón (0.4 × 2.0 cm) con 40.70 ± 0.03 huevos de A. kuehniella. Trichogrammatoidea annulata, T. acacioi, T. brasiliensis, T. bruni, T. pretiosum, y T. soaresi parasitaron huevos de A. kuehniella almacenados hasta 24 días; T. atopovirilia parasitó huevos almacenados hasta 16 días. Trichogramma demoraesi y T. benneti parasitaron huevos almacenados 16 días y T. galloi parasitó huevos almacenados hasta 13 días. El porcentaje de huevos parasitados disminuyo con incrementos al periodo de almacenamiento. Dentro de los parasitoides evaluados T. acacioi parasitó huevos almacenados por periodos más largos y presento los niveles de parasitismo y de emergencia de adultos más altos.

 

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Published

2014-04-05

Issue

Vol. 97, No. 1 (March 2014)

Section

Research Papers

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