Symposium: Use of Pheromones in Tropical Crops: Sex Pheromone Gland of the Navel Orangeworm, Amyelois Transitella (Lepidoptera: Pyralidae) Location, Bioassay and in Vitro Maintenance

Authors

  • Asoka Srinivasan
  • J. A. Coffelt
  • Phillipa Norman
  • Beverly Williams

Abstract

Anatomical studies of the sex pheromone gland of the female navel orangeworm, Amyelois transitella (Walker), revealed the gland to be a broad, chevron-shaped structure located on the ventrolateral surface of the intersegmental membrane between abdominal segments VIII and IX. Support for this finding was obtained by bioassay and gas chromatographic analyses of extracts that were prepared from various portions of the terminal abdominal segments of female moths. Histological examination showed that the gland consists of a single layer of specialized columnar epidermal cells. Sex pheromone glands were obtained from surface-sterilized abdominal segments of 2 to 3-day-old virgin females and subsequently cultured for up to 7 days in either chemically defined or modified Grace's medium. Bioassays of extracted medium in which sex pheromone glands had been maintained indicated that more pheromone was recovered from modified Grace's medium than from the chemically defined growth substrate.

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Published

1986-03-01

Issue

Vol. 69, No. 1 (March 1986)

Section

Literature Review Articles

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