Applications of molecular diagnostics for quality control in rearing of Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae for experimental use

Authors

  • Francisco Javier Flores Gallardo Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico
  • José Luis Hernández Flores Centro de Investigación y de Estudios Avanzados del IPN, Departamento de Ingeniería Genética, Irapuato, Guanajuato, 36824, Mexico
  • Miguel Ángel Ramos López Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico
  • Jackeline Lizzeta Arvizu Gómez Secretaría de Investigación y Posgrado. Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT). Universidad Autónoma de Nayarit, Tepic, Nayarit, 63173, Mexico
  • Víctor Pérez Moreno Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico
  • José Alberto Rodríguez Morales Universidad Autónoma de Querétaro, Facultad de Ingeniería, Querétaro, 76010, Mexico
  • Erika Álvarez Hidalgo Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico
  • Jorge Nuñez Ramírez Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico
  • George H. Jones Emory University, Department of Biology, Atlanta, Georgia 30322, USA
  • Juan Campos Guillén Universidad Autónoma de Querétaro, Facultad de Química, Querétaro, 76010, Mexico

Abstract

Illumina MiSeq sequencing of 16S rRNA genes was applied in a molecular diagnostic analysis of healthy and dead larvae obtained from a Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) colony. The results indicated the presence of Enterococcus (Lactobacillales: Enterococcaceae) with an average abundance of 89.2% and 94.8% in healthy and dead larvae, respectively. Pseudomonas (Pseudomonadales: Pseudomonadaceae) and Acetobacter (Rhodospirillales: Acetobacteraceae) were present in lower abundance. Additional metagenomic results revealed the presence in dead larvae of viral sequences related to Alphabaculovirus in high relative abundance compared with healthy larvae. Additionally, viral sequences of tomato leaf curl Laos virus, Heliothis zea (Boddie) (Lepidoptera: Noctuidae) nudivirus, Clostera anachoreta (Denis & Schiffermüller) (Lepidoptera: Notodontidae) granulovirus, and Tipula oleracea L. (Diptera: Tipulidae) nudivirus were detected. Our results show that molecular detection of pathogenic agents is a useful tool during insect rearing as a quality control to avoid the spread or acquisition of potential pathogens.

 

Sumario

Nuestros resultados demuestran que a través de un diagnóstico molecular en Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), usando secuenciación del 16S rDNA por Illumina MiSeq, en larvas saludables y muertas mostraron una abundancia relativa alta de Enterococcus (Lactobacillales: Enterococcaceae) con un promedio de 89.2% and 94.8% respectivamente. Mientras que Pseudomonas (Pseudomonadales: Pseudomonadaceae) y Acetobacter (Rhodospirillales: Acetobacteraceae) presentaron menor abundancia relativa. Adicionalmente, resultados de metagenomica mostraron en larvas muertas secuen cias de virus relacionados con Alphabaculovirus con una abundancia relativa muy alta comparada con las larvas saludables. Se detectaron secuencias virales relacionadas al Encrespamiento amarillo de la hoja del tomate, Heliothis zea (Boddie) (Lepidoptera: Noctuidae) nudivirus, Clostera anachoreta (Denis & Schiffermüller) (Lepidoptera: Notodontidae) granulovirus and Tipula oleracea L. (Diptera: Tipulidae) nudivirus. Nuestros resultados demuestran que una detección molecular de agentes patogénicos es primordial durante la crianza de insectos como un control de calidad para evitar su dispersión o adquisición.

Key Words: Spodoptera frugiperda; baculovirus; molecular diagnosis; quality control

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Published

2023-07-17

Issue

Vol. 106, No. 2 (June 2023)

Section

Scientific Notes

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