Carrier and vector of Pectobacterium carotovorum subsp. carotovorum and its handling through a base of entomopathogenic fungi in Agave sp.

Authors

  • Teodulfo Aquino-Bolaños Instituto Politécnico Nacional, CIIDIR-Unidad Oaxaca, Hornos 1003, Col. Nochebuena, Santa Cruz Xoxocotlán, Oaxaca, Mexico, C.P. 71230
  • José Antonio Sánchez-García Instituto Politécnico Nacional, CIIDIR-Unidad Oaxaca, Hornos 1003, Col. Nochebuena, Santa Cruz Xoxocotlán, Oaxaca, Mexico, C.P. 71230
  • Yolanda Donaji Ortíz-Hernández Instituto Politécnico Nacional, CIIDIR-Unidad Oaxaca, Hornos 1003, Col. Nochebuena, Santa Cruz Xoxocotlán, Oaxaca, Mexico, C.P. 71230
  • Julián Hernández-Cruz Universidad Tecnológica de la Sierra Sur de Oaxaca, Magnolia s/n Villa Sola de Vega Oaxaca, Mexico, C.P. 71400
  • Carlos I Cortés-Martínez

Abstract

The presence of the carrier and vector of Pectobacterium carotovorum (Jones) (Enterobacteriaceae) was found in Agave potatorum Zucc. (Asparagaceae) (larvae and adults of Scyphopphorus acupunctatus Gyllenhaal [Coleoptera: Curculionidae], larvae of Drosophila melanogaster Macquart [Diptera: Drosophilidae] and Hermetia illucens [L.] [Diptera: Stratiomyidae], and adults of Hololepta sp. [Coleoptera: Histeridae]) in Oaxaca, Mexico. Entomopathogenic fungi have been used as control methods to prevent the development of bacteria under laboratory conditions. After 5 d of inoculation, all the existing insects in the agave plants exhibited 98% of the countable growth of the bacterium, which led to the determination that the 4 species of insects are carriers and vectors of P. carotovorum. According to the final growth of the bacterium and the entomopathogenic fungi, the Beauveria bassiana (Bals.-Criv.) Vuill. (Cordycipitaceae) (E) strain at a concentration of 1.0 × 104 spores per mL showed a difference of 57.2 mm. For this reason, it was considered the best strain to disable the growth of P. carotovorum.

 

Resumen

Se determinaron insectos portadores y vectores de la bacteria Pectobacterium carotovorum presentes en plantas de Agave potatorum Zucc. (larvas y adultos de Scyphopphorus acupunctatus, larvas de Drosophila melanogaster y Hermetia illucens, y adultos de Hololepta sp.), y como métodos de control se utilizaron hongos entomopatógenos para inhibir el desarrollo de la bacteria en condiciones de laboratorio. A los 5 días de la inoculación todos los insectos presentes en las plantas de agave presentaron un 98% de crecimiento contable de la bacteria, por lo que se determinó que las cuatro especies de insectos son portadores y vectores de P. carotovorum. De acuerdo al crecimiento final de la bacteria y los hongos entomopatogenos, la cepa Beauveria bassiana (E) a una concentración de 1.0 × 104 esporas por mL presento una diferencia de 57.2 mm por lo que se considera como la mejor cepa que inhibió el crecimiento de P. carotovorum.

Key Words: agave weevil; Scyphophorus acupunctatus; Beauveria bassiana; Metarhizum anisopliae

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Published

2020-07-09

Issue

Vol. 103, No. 2 (June 2020)

Section

Research Papers

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