Pathogenicity and virulence of Purpureocillium lilacinum (Hypocreales: Ophiocordycipitaceae) on Mexican fruit fly adults

Authors

  • Ricardo A. Toledo-Hernández El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico
  • Jorge Toledo El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico
  • Javier Valle-Mora El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico
  • Francisco Holguín-Meléndez El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico
  • Pablo Liedo El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico
  • Graciela Huerta-Palacios El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico http://orcid.org/0000-0003-2204-8382

Abstract

Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson (Hypocreales: Ophiocordycipitaceae) is a fungus commonly used for controlling nematodes, and also has been reported as an insect pathogen. However, little is known about its effects on insects. Here, the pathogenicity of 9 isolates and the virulence and sublethal effects of 2 isolates were evaluated to control adult Anastrepha ludens (Loew) (Diptera: Tephritidae). The pathogenicity assays demonstrated that the 9 isolates were pathogenic, with mortality percentages ranging from 28.8 to 52.4% and LT50 values were 18 d or more. The strain CFFSUR-A53 was more virulent than CFFSUR-A60, with LC50 values of 7.62 × 106 and 5.2 × 109 conidia per mL, respectively. The isolates reduced the life expectancy of the flies by 65 and 37%, decreased fecundity by 78 and 36%, and reduced egg hatching by 31.4 and 18.5%, respectively.

 

Resumen

Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones & Samson (Hypocreales: Ophiocordycipitaceae) es un hongo comúnmente utilizado en el control de nematodos y ha sido reportado como patógeno de insectos. Sin embargo, son poco conocidos los efectos que causa sobre los insectos. Aquí se evaluó la patogenicidad de nueve aislamientos y la virulencia y efectos subletales de dos aislamientos en adultos de Anastrepha ludens (Loew) (Diptera: Tephritidae). Los ensayos demostraron que los nueve aislamientos fueron patogénicos, y causaron porcentajes de mortalidad que oscilaron entre 28.8 y 52.4% y un TL50 de 18 d o más. La cepa CFFSUR-A53 fue más virulenta que la cepa CFFSUR-A60, con valores de CL50 de 7.62 × 106 y 5.2 × 109 conidios por mL, respectivamente. Ambos aislamientos redujeron en 65 y 37% la esperanza de vida de las moscas, disminuyeron en 78 y 36% la fecundidad de las hembras, y en 31.4 y 18.5% la eclosión de los huevos, respectivamente.

Key Words: entomopathogenic fungi; biological control; Anastrepha ludens, fecundity and fertility

View this article in BioOne https://doi.org/10.1653/024.102.0204

Author Biography

Graciela Huerta-Palacios, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Apdo. Postal 36. 30700, Tapachula, Chiapas, Mexico

Ecología de artópodos y manejo ntegrado de plagas

Investigador titular A

Downloads

Published

2019-07-31

Issue

Vol. 102, No. 2 (June 2019)

Section

Research Papers

License

Copyright for any article published in Florida Entomologist is held by the author(s) of the article. Florida Entomologist is an open access journal. Florida Entomologist follows terms of the Creative Commons, Attribution Non-Commercial License (cc by-nc). By submitting and publishing articles in Florida Entomologist, authors grant the FOJ and Florida Entomologist's host institutions permission to make the article available through Internet posting and electronic dissemination, and to otherwise archive the information contained both electronically and in a hard printed version. When used, information and images obtained from articles must be referenced and cited appropriately. Articles may be reproduced for personal, educational, or archival purposes, or any non-commercial use. Permission should be sought from the author(s) for multiple, non-commercial reproduction. Written permission from the author(s) is required for any commercial reproduction.