Wolbachia infection of Neoceratitis asiatica (Diptera: Tephritidae) in Ningxia

Authors

  • Xiaoxue Wang China Agricultural University http://orcid.org/0000-0002-8637-5422
  • Zhihong Li China Agricultural University
  • Rong Zhang Ningxia Academy of Agriculture and Forestry Sciences
  • Jia He Ningxia Academy of Agriculture and Forestry Sciences
  • Zihua Zhao China Agricultural University
  • Shuhua Wei Ningxia Academy of Agriculture and Forestry Sciences
  • Lijun Liu China Agricultural University

Keywords:

Wolbachia wRi strain, wsp gene, phylogeny

Abstract

Neoceratitis asiatica (Becker) (Diptera: Tephritidae), known as wolfberry fruit fly, is a harmful pest of Lycium barbarum (Solanaceae). Neoceratitis asiatica female adults insert the ovipositor into the peel of L. barbarum and lay eggs, causing reductions in yield and economic loss. The symbioticbacteria Wolbachia spp. have attracted considerable attention and interest by entomologists in recent years. Wolbachia infect many genera of tephritidfruit flies, such as Anastrepha, Bactrocera, Rhagoletis, Dacus, Ceratitis, and Carypomya. Wolbachia can induce complete cytoplasmic incompatibilityin novel hosts, leading to complete suppression of laboratory populations by single releases of infected males, which potentially makes it auseful method for pest management. In this study, the infection of Wolbachia in N. asiatica from the Ningxia region in China was detected based onthe Wolbachia surface protein gene sequence. The neighbor-joining tree showed Wolbachia in wolfberry fruit fly was wRi strain. This research laysthe foundation for further study about Wolbachia in Chinese wolfberry fruit fly, and also provides a basis for the prevention and control of othereconomically important fruit flies using Wolbachia.

 

 

Resumen

Neoceratitis asiatica (Becker) (Diptera: Tephritidae), conocida como la mosca de la fruta de Wolfberry, es una plaga nociva de Lycium barbarum (Solanaceae). Las adultas hembras de Neoceratitis asiatica insertan el ovipositor en la cascara de L. barbarum y ponen huevos, lo que provocauna reducción en el rendimiento y una pérdida económica. La bacteria simbiótica Wolbachia spp. ha atraído una considerable atención e interés por entomólogos en los últimos años. Wolbachia infecta muchos géneros de moscas de la fruta tefrítidas, como Anastrepha, Bactrocera, Rhagoletis, Dacus, Ceratitis, y Carypomya. Wolbachia puede inducir incompatibilidad citoplásmica completa en hospederos noveles, lo que lleva a la supresión completa de las poblaciones de laboratorio por liberaciones únicas de machos infectados, lo que le convierte potencialmente en un método útil para el manejo de plagas. En este estudio, se detectó la infección de Wolbachia en N. asiatica de Ningxia en China en base a lasecuencia del gen de la proteína de la superficie de Wolbachia. El árbol filogenético conjuntando vecinos que se unió mostró que la Wolbachia en la mosca de la fruta de wolfberry es la cepa wRi. Esta investigación sienta las bases para un estudio adicional sobre Wolbachia en la mosca dela fruta de wolfberry chino, y también proporciona una base para la prevención y el control de otras moscas de la fruta económicamente importantesutilizando la Wolbachia.

View this article in BioOne

 

Author Biographies

Xiaoxue Wang, China Agricultural University

Department of Entomology, College of Plant Protection.

Zhihong Li, China Agricultural University

Department of Entomology, College of Plant Protection.

Rong Zhang, Ningxia Academy of Agriculture and Forestry Sciences

The Institute of Plant Protection

Jia He, Ningxia Academy of Agriculture and Forestry Sciences

The Institute of Plant Protection

Zihua Zhao, China Agricultural University

Department of Entomology, College of Plant Protection.

Shuhua Wei, Ningxia Academy of Agriculture and Forestry Sciences

The Institute of Plant Protection

Lijun Liu, China Agricultural University

Department of Entomology, College of Plant Protection.

Downloads

Published

2019-04-27

Issue

Section

Research Papers