DIVERSITY OF ROOT-KNOT NEMATODES PARASITIZING COFFEE IN CENTRAL AMERICA [DIVERSIDAD DE NEMATODOS AGALLADORES ASOCIADOS AL CULTIVO DE CAFÉ EN CENTRO AMÉRICA]

  • L. Villain CIRAD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5, France
  • J. L. Sarah CIRAD-Drs, Avenue Agropolis - TA 179/ 04 34398 Montpellier Cedex 5 France
  • A. Hernández Fundación Procafé, Final Av. Manuel Gallardo Fte a Resid. Monte Sión La Libertad, Santa Tecla, El Salvador
  • B. Bertrand CIRAD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5, France
  • F. Anthony IRD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5 France
  • P. Lashermes IRD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5 France
  • P. Charmetant CIRAD, UMR DAP, TA A 96/03 Avenue Agropolis - 34398 Montpellier Cedex 5 France
  • F. Anzueto ANACAFE, Calle del Café 0-50, Zona 14 Guatemala, Guatemala
  • P. Figueroa ANACAFE, Calle del Café 0-50, Zona 14 Guatemala, Guatemala
  • R. M. D. G. Carneiro EMBRAPA Recursos Genéticos e Biotecnologia, 70849-970, Brasília, DF, Brazil
Keywords: Central America, Coffea arabica, Coffea canephora, electrophoresis, esterase, root-knot nematode

Abstract

The diversity of root knot nematodes parasitizing coffee orchards in Central America was newly assessedthrough a broad regional survey. Populations of Meloidogyne spp. were identified by their esterase phenotype. Elevenesterase phenotypes were observed and nine species identified. Meloidogyne exigua was the most widely distributedwhile M. arabicida, M. arenaria, M. hapla, M. izalcoensis and M. paranaensis appeared to be much more localized.Concerning M. paranaensis, only observed in Guatemala, the two-band esterase phenotypes prevailed. About M.arenaria, the one-band esterase phenotype (A1) was observed for the first time on coffee in one population inGuatemala. A three-band esterase phenotype (A3) was observed in two populations in El Salvador and could belongto M. morocciensis. The presence of M. enterolobii on coffee in Central America was confirmed with one populationfrom Guatemala and another one from Costa Rica as a new report. Based on esterase diagnosis identifications ofM. incognita were made for the first time on coffee in the region: in Costa Rica, El Salvador and Guatemala. Takentogether, the results of the survey revealed a high number of root knot nematode species present on coffee throughoutCentral America.
Published
2013-12-01
Section
ELECTRONIC ARTICLE/ARTICULO ELECTRONICO