ONLINE ONLY; EXTRACTION TECHNIQUES OF THE WHITE-TIP NEMATODE APHELENCHOIDES BESSEYI FROM PADDY SEEDS IN REGULATORY SAMPLES

Authors

  • P. Rajan
  • Arjun Lal

Keywords:

aphelenchoides besseyi, extraction techniques, hydrogen peroxide method, oryza sativa, quarantine, seed certification, seed-transmitted nematode

Abstract

The rice white-tip nematode, Aphelenchoides besseyi, is a plant parasitic and seed-transmitted nematode of phytosanitary concern. Its detection methodology is important for seed certification and quarantine. In search of an effective nematode detection and extraction technique, the efficiency of already known and other appropriately modified procedures were compared in laboratory tests. The hydrogen peroxide (H[sub2]O[sub2]) method for the extraction of endoparasitic migratory nematodes from roots was modified and adapted for the extraction of A. besseyi from infested paddy seeds. Paddy seeds pre-soaked in 5% H[sub2]O[sub2] were spread over wet blotter paper (in 2% H[sub2]O[sub2]), incubated in Petri dishes and rinsed with 2% H[sub2]O[sub2] at regular intervals. The nematodes released from the paddy seeds in the oxygenated aqueous suspension were observed directly. This method resulted in the recovery of the largest number of nematodes which were easily counted after incubation periods of 2, 4, 7 and 10 days.

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Published

2006-06-01

Issue

Section

Articles