ISOLATION OF TWO cDNAS ENCODING A TROPOMYOSIN AND AN INTERMEDIATE FILAMENT PROTEIN FROM THE SOYBEAN CYST NEMATODE HETERODERA GLYCINES
Keywords:heterodera glycines, antibodies, cDNA library, tropomyosin, intermediate filament protein, soybean cyst nematode
AbstractA cDNA expression library of the soybean cyst nematode Heterodera glycines was screened with polyclonal antibodies raised against complete homogenate of Globodera rostochiensis second-stage juveniles (J2). Two cDNAs with complete Open Reading Frames were isolated and sequenced. These cDNAs showed high similarity to genes encoding intermediate filament proteins and tropomyosins of other nematodes, including Caenorhabditis elegans. The sequences were named HG-if 1 (filament protein) and HG-trp 1 (tropomyosin). Considering that IF proteins and tropomyosins are essential for nematode development and show high levels of allergenicity, facilitating the production of antibodies, these molecules have potential as target molecules for the production of plantibodies to control soybean cyst nematode.