DEVELOPMENT OF PCR PRIMERS TO IDENTIFY SPECIES OF ROOT-KNOT NEMATODES: MELOIDOGYNE ARENARIA , M. HAPLA , M. INCOGNITA AND M. JAVANICA
Authors
K. Dong
R. A. Dean
B. A. Fortnum
S. A. Lewis
Keywords:
diagnostic, DNA, identification, Meloidogyne, M. arenaria, M. hapla, M. incognita, M. javanica, PCR, RAPD, root-knot nematode, sequence tagged site, species-specific
Abstract
DNA from twenty-six different single-egg-mass nematode isolates, including seven Meloidogyne arenaria, three M. hapla , eleven M. incognita and five M. javanica , were used to identify species-specific sequence tagged sites. RAPD-PCR was tested to detect species-specific DNA fragments. Nematode isolates of the same species could be grouped unambiguously by most of the polymorphic RAPD patterns. Variations among isolates of each species were also observed, especially within M. arenaria and M. hapla . Potential species-specific DNA fragments from RAPD-PCR were cloned and sequenced. Species- specific PCR primer pairs for M. arenaria , M. hapla , M. incognita and M. javanica were developed.
