Nineteen isolates of Paecilomyces lilacinus from different countries and different regions of Brazil and one isolate of Cylindrocarpon destructans were evaluated in vitro for parasitism of eggs of Meloidogyne javanica. The isolates varied in their ability to colonize eggs. In greenhouse studies with tomato plants, isolates of P. lilacinus and C. destructans reduced the number of galls of M. javanica but differed in effectiveness. The isolates of Paecilomyces lilacinus from France and Italy were the most effective in both in vitro and greenhouse experiments.