Studies on Host-Parasite Relationships of Meloidogyne incognita and Coffea arabica Cv. Borbon

  • Jose A. Negron
  • Nelia Acosta
Keywords: Root-Knot Nematode, Coffee Seedlings, Inoculum Densities, Histopathology


A greenhouse study was conducted to determine the host-parasite relationship of Meloidogyne incognita and Coffea arabica cv Borbon. Two-month-old coffee seedlings were inoculated separately with 4000, 8000, 1600, and 32000 eggs and second-stage juveniles per plant. Uninoculated plants were used as controls. Six months after inoculation, chlorosis and dwarfing were significantly greater in plants inoculated with 32000 eggs and juveniles when compared to other treatments. Height and dry weight of roots and shoots were significantly reduced in inoculated plants. There were no significant differences among plants treated with any population level, but significant differences for all parameters were obtained between any inoculated plants and the control plants. Histological studies of root sections showed multinucleated, thick-walled giant cells surrounding the anterior portion of the females. Mechanical compression of parenchymatous and vascular tissue was observed. Adjacent non-giant ce