A Quantitative Comparison of Some Methods for the Extraction of Nematodes from Roots

Authors

  • R. McSorley
  • J. L. Parrado
  • W. H. Dankers

Keywords:

Techniques, Helicotylenchus multicinctus, Meloidogyne incognita, Hemicriconemoides mangiferae, Pratylenchus brachyurus, Pratylenchus coffeae, Tylenchulus semipenetrans

Abstract

Methods for extracting various species of nematodes from root tissue were compared, including incubation in water, a mist chamber, plastic bags, or Mason jars; maceration of roots followed by centrifugation or suspension in Baermann funnels; or extraction in sodium hypochlorite solutions. Sodium hypochlorite solutions were superior to blender maceration and centrifugation for extracting eggs of Meloidogyne incognita or Tylenchulus semipenetrans. In most cases, blender maceration + centrifugation was superior for extracting migratory stages of T. semipenetrans. Helicotylenchus multicinctus, Pratylenchus spp., or Hemicriconemoides mangiferae. However, numbers extracted by this technique were not significantly different from those obtained in a mist chamber for Pratylenchus spp., nor from those obtained by incubation in plastic bags for T. semipenetrans. Results for extraction of larvae of M. incognita were inconsistent, possibly because of egg hatch during the incubation procedures. Ma

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Published

1984-06-01

Issue

Section

Articles