NEMATICIDES FOR CONTROL OF HETERODERA GLYCINES UNDER IN VITRO AND GREENHOUSE CONDITIONS
DOI:
https://doi.org/10.63965/EVCJ7504Abstract
The soybean cyst nematode (SCN), Heterodera glycines, is a major constraint to soybean production in Brazil. This study evaluated the efficacy of biological and chemical nematicides on egg hatching, second‑stage juvenile (J2) mortality, and nematode reproduction under controlled in vitro and greenhouse conditions. Four in vitro experiments were conducted using eggs and J2 as inoculum, with egg hatching assessed at 7 and 14 days and J2 mortality at 24 and 48 hr after treatment. In greenhouse assays, biological nematicides were applied either as seed treatments or through pre-treatment of nematode inoculum before plant inoculation. Additional experiments assessed both chemical and biological nematicides using eggs as inoculum. Biological nematicides increased J2 mortality in vitro, although their effect on egg hatching was inconsistent. In greenhouse conditions, pre-treating nematode inoculum generally resulted in lower SCN reproduction compared with seed-applied treatments. Both biological and chemical nematicides reduced SCN reproduction, with chemical nematicides, particularly fluopyram, showing the greatest reductions. These results demonstrate that nematicides can suppress SCN under greenhouse and in vitro conditions; however, further field validation is needed to confirm their potential as management tools for soybean production.
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