NEMATODE EXTRACTION EFFICIENCY IS INFLUENCED BY SOIL DEPTH AND INOCULATION LEVEL
DOI:
https://doi.org/10.63965/BLNJ3327Abstract
There is a need for evaluating nematode abundances from different soil types by optimizing extraction efficiency (i.e., nematode recovery) because soil texture, pH, and soil organic matter change as soil depth increases. Therefore, the extraction efficiency of a modified Baermann technique on soil recovered from different depths (0‑30 cm, 30-60 cm, 60-90 cm, and 90-120 cm) at a long-term peanut-cotton rotation study in Quincy, FL, USA was examined. This was accomplished by evaluating the relative efficiency of reniform (Rotylenchulus reniformis) and free-living nematode extraction from these different soil depths based on the addition of known quantities of nematodes to microwave-treated soil. Depth and nematode inoculation levels both significantly affected extraction efficiency of reniform and free-living nematodes, but in different ways. For reniform nematode, extraction efficiency ranged from 36% at the deepest depth (90‑120 cm) and increased to 47% at the shallowest depth (0-30 cm). Yet, for free-living nematodes, extraction efficiency ranged from 25% at the deepest depth and increased to 36% at the shallowest depth. Reniform nematode extraction efficiency was greatest (39%) with the lowest inoculation rate (i.e., 1,000 reniform nematodes per 100 cm3 soil), while trends with free-living nematode extraction efficiency were inconsistent. This work highlights the need to understand that true nematode population densities may be underestimated due to soil type and correction factors may need to be implemented for diagnostic and management purposes.
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