INFLUENCE OF EXTRACTION PROCEDURES FROM ROOT SAMPLES ON THE RECOVERY AND INFECTIVITY OF PRATYLENCHUS ZEAE AND HIRSCHMANNIELLA ORYZAE

Abstract

The efficiency of three extraction techniques - Baermann funnel (BD, maceration-filtration (M-F), and mistifier (Mi) - to extract Pratylenchus zeae and Hirschmanniella oryzae from rice roots and their influence on the infectivity of P. zeae were tested. M-F and Mi methods were the most efficient techniques for extracting P. zeae and H. oryzae, respectively. When comparing infestation of rice plant roots using infested roots or nematodes obtained by Bf, M-F, or Mi methods as sources of inoculum, infectivity of P. zeae was strongly decreased by these extraction techniques. However, more P. zeae were able to infest rice roots when they were obtained using the Mi technique. The duration of extraction also affects the infectivity of P. zeae. When estimating root infestation by migratory endoparasitic nematodes, it may be necessary to use different techniques to extract different nematodes. The effect of the method by which the nematodes have been obtained must also be considered in assessing their infectivity.