EFFECT OF CRUCIFEROUS TISSUE CULTURES AND MEDIA ON THE PENETRATION OF HETERODERA AVENAE IN WHEAT IN VITRO

Abstract

Excised roots and callus cultures of wheat and crucifers were raised on synthetic media. Differences in nematode behaviour were observed with combinations of wheat and crucifers. MS solidified agar medium was better for propagation and maintenance of excised roots. Eruca sativa suppressed penetration of roots by juveniles of Heterodera avenae significantly. Use of gnotohiotic technique for the establishment of antagonism of crucifers to cereal cyst nematode on wheat is described.

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