GENETIC VARIATION AND PATHOTYPE RESPONSE IN POTATO CYST-NEMATODES FROM CYPRUS

Abstract

Thirty-six samples of potato cyst-nematodes (PCN) from Cyprus (Xylophagou and Troodos regions) were examined for pathotype composition and genetic variation using differential potato clones and isozyme analysis. Isoelectric focusing (IEF) analysis revealed Globodera rostochiensis to be predominant in the samples, with 32 out of 36 populations consisting of this species. Of the remaining four samples, three were G. rostochiensis/G. pallida mixtures and one was pure G. pallida. These analyses were confirmed in the pathotyping tests and subsequent electrophoretic analysis of cysts produced in these tests.