Morphological and Morphometrical Characterization of Meloidogyne incognita from Different Host Plants in Four Districts of Punjab, India

Abstract

The population of M. incognita, the root knot nematode (RKN) was found infesting five different host plants (okra,banana, sunflower, bottle gourd, and brinjal) out of 24 examined from four districts of Punjab, India (Gurdaspur, Ludhiana, Patiala,and Hoshiarpur). Morphological and morphometrical characterization indicated that in the case of mature female, the characters ofbody length and width, neck length, ratio ‘a’, anus to tail terminus (ATT), interphasmid distance (IPD), and perineal pattern wererecorded as stable characters. These taxonomic characters can be reliable for identification. All characters of second-stage juvenile( J2) such as body length, stylet length, head to median bulb length (H-MB), distance from median bulb to excretory pore (MB-EP),tail length, anal body width (ABW), and ratios C and C’ were highly variable. Analysis of interpopulation morphometric characters ofmature female of M. incognita, namely, body length, width, and ratio ‘a’ were moderately variable characters (CV 0.26% to 20%) andstylet length, neck length, length of median bulb (LMB), and width of median bulb (WMB) were highly variable (CV 1.0% to 36.1%).In the perineal pattern, the two characters ATT and IPD were moderately variable (CV 8.8% to 17.6%) and two characters, anus tovulval slit (AVS) and length of vulval slit (LVS), were highly variable (CV 2.1% to 40.5%). In J2, body length, stylet length, H-MB,MB-EP, ABW, tail length, ratios C, and C’ were highly variable characters (CV > 12%).