Isolation of a Repeated DNA Probe Showing Polymorphism among Meloidogyne incognita Populations
Abstract
Several Meloidogyne incognita geographic populations were characterized by analysis of the restriction fragment length polymorphisms (RFLP) obtained after digestion of their total DNA and hybridization with a [32P]-labeled probe. The probe consisted of a 1.7-kb-repeated DNA sequence, isolated from a M. incognita genomic library, that hybridized to multiple BamH I fragments in the genome of each isolate. The patterns showed sufficient polymorphism to enable the accurate differentiation of all the populations tested. Key words: DNA, hybridization, intraspecific differentiation, Meloidogyne incognita, repetitive DNA.Downloads
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