Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes
B. A. Jaffee
Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced in cultures of transformed potato roots increased with increasing nematode inoculum levels, whether inoculum was dispersed eggs or juveniles. Females appeared smaller, produced fewer eggs, and were found in coalesced galls at the higher inoculum levels. The ratio between the final and initial population decreased sharply as the juvenile inoculum increased. The second-stage juvenile was preferred to dispersed eggs or egg masses for inoculation of tissue culture systems because quantity and viability of inoculum were easily assessed. Meloidogyne javanica reared on transformed root cultures were able to complete their life cycles on new transformed root cultures or greenhouse tomato plants. Key words: Agrobacterium rhizogenes, bindweed, carrot, gnotobiotic, hairy root pathogen, inoculum level, lima bean, Meloidogyne javanica, monoxenic, nematode Stage, potato, root-knot nematode, tissue culture, tomato.
All material published by the Society of Nematologists (SON), except for papers prepared by United States and Canadian government employees, is copyrighted and protected under the U.S. copyright law. Under the Copyright Act of 1976, the term of copyright for materials registered by an organization is 75 years from the date first published. Before publishing any manuscript, SON requires that authors transfer full and complete ownership of any copyright to SON by signing a JON Page Charge/Copyright Form (.pdf). SON then registers the copyright. Subsequent use of published materials requires written permission from the SON and may be obtained by contacting the current Editor-in-Chief and state where and how the material will be used.
The author warrants that the article is an original work not published elsewhere in whole or in part, except in abstract form, and that the author has full power to make this grant. If portions of the article have been published previously, then the author warrants that permission has been obtained from the copyright holder and the author will submit a copy of the permission release with this copyright transfer form.
SON shall claim no proprietary right other than copyright. Authors and coauthors retain the right to revise, adapt, modify, or otherwise use all or part of the article in future works of the author(s), such as press releases, lectures, and reviews, provided that all such use is for the personal noncommercial benefit of the author(s). All patent rights are retained by the author(s).