Inoculation Method for Studying Early Responses of Glycine max to Heterodera glycines
Abstract
An inoculation technique was developed for studying molecular responses of soybean to the soybean cyst nematode (Heterodera glycines). Effect of inoculum age (0-7 days after eggs were released from cysts) and inoculation site (meristem, elongation, or differentiation zone) on infection were tested on four soybean genotypes. Two genotypes (PI 437654 and cv. Peking) were resistant and two (cv. Essex and cv. Hutcheson) were susceptible to race 3 of H. glycines. Inoculum consisting of second-stage juveniles (J2) was prepared by gently agitating nematode eggs at 75 revolutions per minute at 28ºC for various intervals. Infection rates were monitored cytologically. The most consistent infection rate was obtained with 48-hour-old inoculum containing more than 80% J2. More than 100 juveniles/root were observed after inoculation with the 48-hour-old inoculum placed at the root elongation zone, in both resistant and susceptible soybeans. Horizontal orientation of roots during inoculation, the use of concentrated J2 inoculurn (500 J2 in 125 [mu]l/root), and restriction of inoculum to the root elongation zone facilitated synchronous root infection. Key words: Glycine max, hatching, Heterodera glycines, infection, inoculation, method, nematode, root elongation zone, soybean, soybean cyst nematode.Downloads
Published
Issue
Section
License
Copyright and Permissions
All material published by the Society of Nematologists (SON), except for papers prepared by United States and Canadian government employees, is copyrighted and protected under the U.S. copyright law. Under the Copyright Act of 1976, the term of copyright for materials registered by an organization is 75 years from the date first published. Before publishing any manuscript, SON requires that authors transfer full and complete ownership of any copyright to SON by signing a JON Page Charge/Copyright Form (.pdf). SON then registers the copyright. Subsequent use of published materials requires written permission from the SON and may be obtained by contacting the current Editor-in-Chief and state where and how the material will be used.
The author warrants that the article is an original work not published elsewhere in whole or in part, except in abstract form, and that the author has full power to make this grant. If portions of the article have been published previously, then the author warrants that permission has been obtained from the copyright holder and the author will submit a copy of the permission release with this copyright transfer form.
SON shall claim no proprietary right other than copyright. Authors and coauthors retain the right to revise, adapt, modify, or otherwise use all or part of the article in future works of the author(s), such as press releases, lectures, and reviews, provided that all such use is for the personal noncommercial benefit of the author(s). All patent rights are retained by the author(s).
