DNA Sequences from Formalin-Fixed Nematodes: Integrating Molecular and Morphological Approaches to Taxonomy

Authors

  • W. Kelley Thomas
  • J. T. Vida
  • Linda M. Frisse
  • Manuel Mundo
  • James G. Baldwin

Abstract

To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses. Key words: DNA sequence, formalin, microdissection, molecular biology, nematode, polymerase chain reaction, taxonomy.

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Published

1997-09-15

Issue

Vol. 29, No. 3 (September 1997)

Section

Articles

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