A Centrifugation Method for Attaching Endospores of Pasteuria spp. to Nematodes

  • T. E. Hewlett
  • D. W. Dickson


Attachment of relatively low numbers of endospores from two isolates of Pasteuria spp. to several species of nematodes was consistently achieved in 2-5 minutes with a centrifugation technique. The rate of attachment of Pasteuria penetrans at 10[sup4] endospores/0.1 ml/tube to second-stage juveniles (J2) of Meloidogyne javanica, M. incognita race 1, M. incognita race 3, and M. arenaria races 1 and 2 in two tests averaged 4.4, 5.2, 0.1, 0.3, and 0 endospores per J2, respectively. The rate of attachment Pasteuria sp. at 10³ endospores/0.1 ml/tube to individuals of Hoplolaimus galeatus, Belonolaimus longicaudatus, M. arenaria race 1, M. javanica, and M. incognita race 1 in two tests averaged 0.8, 0.04, 0, 0, and 0 endospores per nematode, respectively. The rate of attachment of P. penetrans to M. javanica at 10³, 10[sup4], or 10[sup5] endospores/0.1 ml/tube from two tests averaged 1.0, 5.7, and 28.3 endospores per J2, respectively. All of the J2 had endospores attached following centrifugation in tubes with 10[sup4] and 10[sup5] endospores/0.1 ml/tube. Key words: bacterium, biological control, centrifugation, endospore, Meloidogyne arenaria, M. incognita, M. javanica, method, nematode, Pasteuria penetrans, Pasteuria sp.