Specific Gravity of Spores of Pasteuria penetrans and Extraction of Spore-filled Nematodes from Soil
AbstractThe specific gravity of spores of Pasteuria penetrans collected from Meloidogyne arenaria was found to be around 1.28. Increasing the sucrose concentration used for the extraction of Pratylenchus scribneri from a specific gravity of 1.14 to 1.26 led to the recovery of higher numbers of specimens filled with spores of Pasteuria sp. (P = 0.05). The numbers of spore-filled specimens of Hoplolaimus galeatus recovered from field soil were not affected by the concentration of the sucrose solutions. Belonolaimus longicaudatus was recovered from field soil in greater numbers in sucrose solutions with specific gravities of 1.22 and 1.26 than with a specific gravity of 1.14 (P = 0.05). Key words: Belonolaimus longicaudatus, biological control, centrifugal flotation, extraction, Hoplolaimus galeatus, nematode, Pasteuria spp., Pratylenchus scribneri, specific gravity.
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