DNA Complexity of the Root-knot Nematode (Meloidogyne spp.) Genome

  • E. C. Pableo
  • A. C. Triantaphyllou


Cot curves derived from renaturation kinetics of sheared denatured DNA indicated that the genome of six populations representing the four most common root-knot nematode species (Meloidogyne incoffnita, M. arenaria, M. javanica, and M. hapla) is composed of 20% repetitive and 80% nonrepetitive sequences of DNA. Cot curves were almost identical, indicating that all populations had a haploid genome of approximately the same size. Calculations from an average Cot curve gave an estimate of 0.51 x 108 nucleotide base pairs for the haploid genome of the four Meloidogyne species. This genome is about 12-13 times larger than the genome of the E. coli strain used as a control. Key words: Cot analysis, DNA, Meloidogyne, repetitive DNA, root-knot nematode, unique DNA.