Genetic Analysis of Esterase Polymorphism in the Soybean Cyst Nematode, Heterodera glycines
Abstract
The genetic basis of esterase polymorphism in Heterodera glycines was investigated through controlled matings and analysis of F[sub1] and F[sub2] progeny. Three nematode lines, each fixed for a different esterase phenotype, were isolated and purified through repeated directional selection and inbreeding. Each phenotype was characterized by its distinct pair of closely spaced bands of esterase activity. Single-female single-male crosses were conducted according to a modified agar-plate mating technique. F[sub1] progeny were homogeneous, exhibiting both parental esterase phenotypes (codominant heterozygotes) but no hybrid bands. Approximately 1,500 F[sub2] progeny segregated in a 1:2:1 ratio for expression of the esterase phenotypes of the female parental line, the heterozygote, and the male parental line. Apparently the three esterase phenotypes correspond to three codominant alleles of a single esterase locus. Reciprocal crosses gave similar results, suggesting no maternal inheritance. Key words: esterase, genetics, Glycine max, Heterodera glyci.nes, hybridization, inheritance, isozyme, soybean cyst nematode.Downloads
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