Screening Soybean for Resistance to Heterodera glycines Ichinohe Using Monoxenic Cultures

  • J. A. Lauritis
  • R. V. Rebois
  • L. S. Graney


A simple, rapid, and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of Glycine max (L.) Merr., cultured on a holidic agar medium, were inoculated with axenic second-stage larvae of Heterodera glyciues Ichinohe, Race 3. A clear separation of susceptible and resistant cultivars, based on numbers of mature female nematodes present after 3 wk at 25 C, was observed. The method described should aid researchers in the evaluation of the host response to infection by H. glycines.